Protein Info for ABZR87_RS06250 in Ralstonia sp. UNC404CL21Col

Annotation: hydroxymethylglutaryl-CoA lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 7 to 278 (272 residues), 173 bits, E=4.7e-55

Best Hits

Swiss-Prot: 59% identical to LIUE_PSEAE: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase (liuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 98% identity to rpi:Rpic_0114)

MetaCyc: 59% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• ketogenesis (4/5 steps found)
• mevalonate degradation (1/2 steps found)
• cis-geranyl-CoA degradation (3/9 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Geraniol degradation
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4

Protein Sequence (309 amino acids)

>ABZR87_RS06250 hydroxymethylglutaryl-CoA lyase (Ralstonia sp. UNC404CL21Col)
MSMPNFVKVVEVGPRDGLQNEKSPVSTDTKVELVNRLSDAGFVNVEAASFVSPKWVPQMA
DGADVMARIQRRPGTLYSVLTPNMKGFEGAAAAGADEIVIFSAASEAFAQKNINCSIAES
IERFVPVAKAAKGAGIRVRGSISCSLGCPYQGEVPVSSVLDVIKRMADLGCDEIDIADTI
GVGTAGKVKTVMEAAAATFPIDRLSGHFHDTYGQALANILASLEVGIAIFHASVAGLGGC
PYAKGATGNVATEDVLYLLHGLGIRTGIDLDKVVLAGDFISQAIGRPTASRAGRALLLKL
QDQIAQACI