Protein Info for ABZR87_RS06050 in Ralstonia sp. UNC404CL21Col

Annotation: fused isobutyryl-CoA mutase/GTPase IcmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 24 to 137 (114 residues), 61.3 bits, E=9.6e-21
• PF02310: B12-binding
  • amino acids 26 to 129 (104 residues), 52.1 bits, E=1.2e-17
• PF03308: MeaB
  • amino acids 178 to 410 (233 residues), 142.8 bits, E=2.1e-45
• PF02492: cobW
  • amino acids 208 to 377 (170 residues), 32.8 bits, E=1.1e-11
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 545 to 1094 (550 residues), 382.1 bits, E=3.4e-118
• PF01642: MM_CoA_mutase
  • amino acids 570 to 1088 (519 residues), 423.8 bits, E=1.8e-130

Best Hits

Swiss-Prot: 85% identical to ICMF_CUPMC: Fused isobutyryl-CoA mutase (icmF) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K11942, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 75% identity to azo:azo0696)

Predicted SEED Role

"B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (2/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (6/13 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (1095 amino acids)

>ABZR87_RS06050 fused isobutyryl-CoA mutase/GTPase IcmF (Ralstonia sp. UNC404CL21Col)
MTDLSAVRAADPTPASAVAPVRNKVRFVTAASLFDGHDASINIMRRILQSMGCEVIHLGH
NRSVDEVVNAALQEDVQGIAVSSYQGGHVEYFKYMIDALRARGGEHIQVFGGGGGVIVPT
EIRELQDYGVARIYSPEDGQRMGLAGMIADMVQRCDIDLSVYAPTTLEPVSIGDRRALAQ
LITALESGRIDPALRDAVRAAAAATPTPVVGITGTGGAGKSSLTDELIRRFRLDQRDTLR
IAVISIDPSRRKSGGALLGDRIRMNAINHPNIFVRSLATREASREISDALPDVIAACRAG
GFDLVIVETSGIGQGDAAIVPHVDLSLYVMTPEFGAASQLEKIDMLDFADLVAINKFDRK
GAQDAWRDVAKQVQRNREQWLARPEDMPVFGTQASHFNDDGVTALYHALADRLTERGMAL
AERTLPRPAGKCSTSHDAIVPPARVRYLAELADTVRGYHRRVDAQSRLARERQQLRASHR
MLEQAGATVQTLSALDKQAAERDAQLGAAERKLLAMWPDLRRAYSGDEYVVKIRDKEIRT
QLTHTTLSGTTIRKVVLPPYEDDGEVLRWLMRENVPGSFPYTAGVFAFKRENEDPTRMFA
GEGDAFRTNRRFKLVSEGMEAKRLSTAFDSVTLYGEDPALRPDIYGKVGNSGVSIATLDD
MEVLYDGFDLTSPATSVSMTINGPAPTILAMFMNTAIDQNLARFRADNQREPTQDEEAKI
RAWVLQNVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIQEYFVHHQVRNFYSVSISGY
HIAEAGANPISQLAFTLANGFTYVEAYLARGMHIDDFAPNLSFFFSNGMDPEYSVLGRVA
RRIWAVTLRDKYGANERSQKLKYHVQTSGRSLHAQEIAFNDIRTTLQALIAIYDNCNSLH
TNAYDEAITTPTAESVRRALAIQLIINREWGLAKCENPNQGSFVIDELTDLVEEAVLQEF
ERIAERGGVLGAMETGYQRGKIQEESMLYEQRKHDGSLPIIGVNTFRNPDAESVVPPHIE
LARSSEEEKQSQLARLEAFHARHAREAPAMLQRLQRAVIDDQNVFAVLMDAVRVCSLGQI
THALFEVGGQYRRNM