Protein Info for ABZR87_RS05315 in Ralstonia sp. UNC404CL21Col

Annotation: efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1050 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 341 to 360 (20 residues), see Phobius details amino acids 367 to 387 (21 residues), see Phobius details amino acids 393 to 414 (22 residues), see Phobius details amino acids 437 to 460 (24 residues), see Phobius details amino acids 474 to 498 (25 residues), see Phobius details amino acids 539 to 559 (21 residues), see Phobius details amino acids 872 to 890 (19 residues), see Phobius details amino acids 897 to 917 (21 residues), see Phobius details amino acids 924 to 946 (23 residues), see Phobius details amino acids 971 to 992 (22 residues), see Phobius details amino acids 1005 to 1029 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1027 (1027 residues), 1254.1 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1037 (1037 residues), 1614.9 bits, see alignment E=0 PF03176: MMPL" amino acids 336 to 500 (165 residues), 37.5 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 58% identical to ACRF_ECOLI: Multidrug export protein AcrF (acrF) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 66% identity to adn:Alide_0452)

MetaCyc: 55% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1050 amino acids)

>ABZR87_RS05315 efflux RND transporter permease subunit (Ralstonia sp. UNC404CL21Col)
MAKFFIDRPVFAWVLALFIILAGAISITQLPISQYPTIAPPSVIITATYPGANAKTLDES
VTSIIEQEMNGADGLLYMESVSQGGNGQAQITVTFKAGTDPALAQVDVQNRLKRVEARLP
SQVTQQGVQVDKARSNFLLFATLASTDGKMDPVALGDYISRNVLNEIKRVPGVGQAVLFG
TERAMRVWIDPAKLVGYKLTPTDVYNAIRAQNALVSAGTIGDLPSVSDQPLAATVVVEGQ
LTTTEQFGNIVLVAKPDGSLVRVKDVARLELGGQTYSTSARINGQPISAIGVQLSPTGNA
LGTAKAVKAKLEELSKYFPAGVEYKVPYDTSKFVQISIEEVVKTLFEAMVLVFLVMLLFL
QNIRYTLVPSIVVPISLLGAFAVMNAMGFSINVLTMFGLVLAIGILVDDAIVVVENVERI
MAEEGLPPREATRKAMGQITGAIIGITLVLMAVFIPMAFFSGSVGAIYRQFSLSMVSSIF
FSALMALTLTPALCSTLLKPIEKGSHHEKKGFFGWFNRMFSTTTNRYQSFVERILKKTFR
YMVIYAVLIAAVVFLFLRLPSSFLPTEDQGYIVTNIQLPPGASANRTLEVIKQVENYYKQ
EKAVENIVAVQGFSFSGNGPNAGIVFTPFKDWSERKGKDQTADAVAGRAFGALFGGIRDA
IVFPLNPPPIPELGNATGFTFRLQDRAGLGHDALMAARNQLMGMAAQSKIITGMRPEGLE
DSPQYQVDIDREKANALGVSFTDINAVLSTALGSAYANDFPNYGRQQRVIVQADKINRMQ
PEDIMNLYVRNSQGNMVPMSTFAKGHWIVGPVQLVRYNGYPAVRLSGAAAPGASSGDAMA
EMERLAAKLPAGIGYEWTGQSLQEKASGSQAPALYALSLLAVFLVLAALYESWSIPAAVI
LVVPLGVLGALLGVTLRAMPDDVYFKVGLIAVVGLSAKNAILIIEFAKDLQAQGKGLIEA
TLEAVHLRFRPIIMTSFAFILGVLPLAIATGASSASQRAIGTGVMGGMFTATVLAVVLVP
VFFVVVRRIFKGSERQRRLDAAHRPLDEEI