Protein Info for ABZR87_RS05280 in Ralstonia sp. UNC404CL21Col

Annotation: phosphoenolpyruvate carboxykinase (GTP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 622 PF17297: PEPCK_N" amino acids 26 to 245 (220 residues), 349.3 bits, see alignment E=8.4e-109 PF00821: PEPCK_GTP" amino acids 249 to 619 (371 residues), 552.3 bits, see alignment E=3.9e-170

Best Hits

Swiss-Prot: 99% identical to PCKG_RALPJ: Phosphoenolpyruvate carboxykinase [GTP] (pckG) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01596, phosphoenolpyruvate carboxykinase (GTP) [EC: 4.1.1.32] (inferred from 99% identity to rpf:Rpic12D_3416)

MetaCyc: 54% identical to cytosolic phosphoenolpyruvate carboxykinase (GTP) (Homo sapiens)
Phosphoenolpyruvate carboxykinase (GTP). [EC: 4.1.1.32]; 4.1.1.32 [EC: 4.1.1.32]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (622 amino acids)

>ABZR87_RS05280 phosphoenolpyruvate carboxykinase (GTP) (Ralstonia sp. UNC404CL21Col)
MNQPVMQGVPALNVPDYVKHPRLIAWVGEVAALTKPERIVWCDGSQEEYDRLCAEMVAAG
TMKQLNPAKRKNSYLALSDPSDVARVEDRTFICSQKKEDAGPTNNWTAPAEMRQTLNGLF
DGSMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRAVYDVLGTDGDF
VPCVHTVGKPLAAGEKDVPWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDQGWLAEHMLILGVTSPEGKTYHVAAAFPSACGKTNFAMLIPPAGLNGWKVTTIG
DDIAWIKPRQDANGKTRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDD
GDVWWEGMTDTPPAHLIDWQGQDWTPETAKETGRKAAHPNARFTAPAAQCPSIDPEWDNP
AGVPIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAML
PFCGYNMADYFAHWLKLGEKLAESGATLPKIFCVNWFRKDENGKFVWPGFGENMRVLKWM
VDRIEGQAQGDEHVFGVSPRYEELSWDGLDFTAEQFAKVISLDAAAWKEELALHDELFTK
LAHGLPQALPDAKTRLEERLGG