Protein Info for ABZR87_RS05070 in Ralstonia sp. UNC404CL21Col

Annotation: class I SAM-dependent rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 PF17785: PUA_3" amino acids 4 to 67 (64 residues), 73.5 bits, see alignment E=3.4e-24 PF02475: Met_10" amino acids 130 to 270 (141 residues), 26.5 bits, see alignment E=1.9e-09 PF10672: Methyltrans_SAM" amino acids 173 to 367 (195 residues), 100.7 bits, see alignment E=3e-32 PF03602: Cons_hypoth95" amino acids 221 to 309 (89 residues), 33.1 bits, see alignment E=1.6e-11 PF13847: Methyltransf_31" amino acids 224 to 356 (133 residues), 27.3 bits, see alignment E=9.8e-10 PF09445: Methyltransf_15" amino acids 225 to 304 (80 residues), 24.2 bits, see alignment E=7.9e-09

Best Hits

Swiss-Prot: 50% identical to RLMI_SHEAM: Ribosomal RNA large subunit methyltransferase I (rlmI) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 100% identity to rpf:Rpic12D_3375)

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (397 amino acids)

>ABZR87_RS05070 class I SAM-dependent rRNA methyltransferase (Ralstonia sp. UNC404CL21Col)
MQTLILKPGKERSLLRRHPWIFANAIDRVEGKPASGATVIVRAHNGQFLARAAFSPVSQI
RARVWSFDESEPVDHAFFKRRIAAAVAYRRAWVHDTDAVRLVMGEADGLPGLIVDQYGTG
DAGQLVCQFSAAGVEHWKQAIVAALIKETGCPNVYERSDVSVREREGLEQMTGVLAGAEP
PADLSATEHGVRYYVDVREGHKTGFYVDQRDNRALVGALARDRDVLNCFCYTGGFSLAAL
KGGARSVVSIDSSGEALAVAERNVTLNGFDPARAEWLDADVFKSLRNFRAEGRQFDLIVL
DPPKFAASAQQVDRAARAYKEINLVGMQLLRPGGLLFTYSCSGAIDADLFQKIVAGAAAD
AKVTARIQRRLAGGVDHPLLTSFPEGEYLKGLLLQVG