Protein Info for ABZR87_RS04995 in Ralstonia sp. UNC404CL21Col

Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876 TIGR01056: DNA topoisomerase III" amino acids 3 to 615 (613 residues), 570.1 bits, see alignment E=3.9e-175 PF01751: Toprim" amino acids 5 to 130 (126 residues), 33 bits, see alignment E=1.2e-11 PF01131: Topoisom_bac" amino acids 154 to 595 (442 residues), 390.1 bits, see alignment E=2.8e-120 PF07282: OrfB_Zn_ribbon" amino acids 600 to 650 (51 residues), 27.2 bits, see alignment 5.9e-10 PF13342: Toprim_Crpt" amino acids 647 to 707 (61 residues), 63.2 bits, see alignment 3.6e-21 amino acids 754 to 814 (61 residues), 66.8 bits, see alignment 2.7e-22

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 81% identity to bpy:Bphyt_0306)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (876 amino acids)

>ABZR87_RS04995 DNA topoisomerase III (Ralstonia sp. UNC404CL21Col)
MSKALIIAEKPSVAADIARALGGFTKHDEYFESDDYVLSSAVGHLVEIAAPDEYEVKRGK
WSFAHLPVIPPHFDLRPIAKSESRLKVLTRLIKRKDVTSLINACDAGREGELIFRLIAQH
ANTKLPVKRLWLQSMTPQSIRDGFAKLRSDEEMIPLADAARCRSEADWLVGINGTRAMTA
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIKRFVQRDYWEVRAEFIAAAGLYEGRWFDP
KFKKNEFDPEQRDSRLWSEAEAKSIVAACRDKPGSVTEESKPSTQMSPALFDLTSLQREA
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDSRALPEDYMGTVKQTLDMLGESSPNYLTH
AKKILANQWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVRRFLAVFFPA
AEYQVTTRITEVAGHHFKTEGKVLVNPGWLVVYGREAQGEDANLVPVAKDEKVKTDKVEP
VGLTTKPPARYNEATLLSAMEGAGKLVDDDALREAMAGKGLGTPATRAAIIEGLLTEKYL
VREGRELIPTAKAFQLMTLLRGLGVEELTQAELTGGWEHKLSLIERGQLERDEFMREIAQ
MTQQIVKRAKEYDSDTIPGDYATLSTPCPQCGHVVKENYRRFACSHCDFSISKIPGGRQF
ELNEVEELLTNKTIGPLQGFRSKMGRPFAAILKLAQEDGHWKMEFDFGQNDEDDGEPVDF
SGQESVGHCPKCNGNVYEHGLKYVCENAVAQPKTCDFTTGKIILQQEISREQLAKLLTEG
KTDLLTGFKSARTGRNFKAFLVKQPDGKIGFEFEPRGEGKGKPGAKTAAKKTTAAAKTPA
AKKAPAKTASRTAAKKAPARKKAAEAEADEESTSET