Protein Info for ABZR87_RS04785 in Ralstonia sp. UNC404CL21Col

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 25 to 101 (77 residues), 108.3 bits, E=1.4e-35
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 140 to 599 (460 residues), 709.9 bits, E=4.8e-218
• PF01964: ThiC_Rad_SAM
  • amino acids 142 to 596 (455 residues), 621 bits, E=1e-190

Best Hits

Swiss-Prot: 95% identical to THIC_RALSO: Phosphomethylpyrimidine synthase (thiC) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 98% identity to rpi:Rpic_3642)

MetaCyc: 75% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (631 amino acids)

>ABZR87_RS04785 phosphomethylpyrimidine synthase ThiC (Ralstonia sp. UNC404CL21Col)
MPQAKNAPAASFDSLQSELDQKFAYPASSKTYIVGSRPDIRVPMRTIKQTATRTDKGEML
NPPVPVYDTSGPYSDPDVHIDLKAGLAPLRAKWIDERGDTEILSGLSSEYGQTRANDPAT
AHLRFSHISKPRRAKAGANVSQMHYARRGIITPEMEYVALRESLNLQALQDKPEYRQLLK
QHPGFSYGANLPQRPEDITPEFVRAEVAAGRAIIPANINHTELEPMAIGRNFRVKINGNL
GNSAVTSSLAEEVEKMVWAIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQAL
DKTGGVAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTADRITGIVSRGGSIMA
KWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDAAQFGELHTLGELT
KKAWEHDVQVMIEGPGHVPLQRVQANMDEELKHCFEAPFYTLGPLVTDIAPGYDHITSGI
GAANIGWYGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNAL
SKARFEFRWEDQFNLGLDPEKARAFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDY
AATLDSNATAAVSGMEEKSIEFRKSGSKIYS