Protein Info for ABZR87_RS03220 in Ralstonia sp. UNC404CL21Col

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF12146: Hydrolase_4" amino acids 69 to 308 (240 residues), 45.7 bits, see alignment E=8.1e-16 PF00561: Abhydrolase_1" amino acids 73 to 181 (109 residues), 78 bits, see alignment E=1.4e-25 PF12697: Abhydrolase_6" amino acids 74 to 313 (240 residues), 80.7 bits, see alignment E=3.9e-26

Best Hits

KEGG orthology group: None (inferred from 99% identity to rpf:Rpic12D_3014)

Predicted SEED Role

"3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>ABZR87_RS03220 alpha/beta hydrolase (Ralstonia sp. UNC404CL21Col)
MNTATTGELFASPPRANLLNVARAMLRPGRIGLRSIGRDVLIARYAKPQSRYLPLMGTRV
HYTDEGAANAEGTLLLIHGFGASLHTWDGVLPQLTRRYRVIRLDLPPFGITGPLRDAQGR
PRTMELPLYRDFIDAFVDSLGLSKLTLIGNSLGGMVSWDFAVRHPGRVEKLVLIDSAGFP
MKLPIYIDLFNHIGVRMTSPWMLPEGIIRAATRDVYGDPSRVSEPTLRRYADFFYADGAR
QAIGKMVPKFRFDDVDTSGLASIRVPTLILWGQRDRWIPPAHAGEFARRIPGATLRMYPA
LGHIPMEEDPVRVGTDLCAFLDQGRASTRLSETTFQENRP