Protein Info for ABZR87_RS02570 in Ralstonia sp. UNC404CL21Col
Annotation: glutamate synthase-related protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00284, glutamate synthase (ferredoxin) [EC: 1.4.7.1] (inferred from 75% identity to aav:Aave_1008)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle II (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamate biosynthesis V (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- nitrogen remobilization from senescing leaves (6/8 steps found)
- GABA shunt II (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.7.1
Use Curated BLAST to search for 1.4.1.13 or 1.4.7.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1573 amino acids)
>ABZR87_RS02570 glutamate synthase-related protein (Ralstonia sp. UNC404CL21Col) MEQHSFPITPSAPSAQGLYDPTNEHDACGVGFVAHIKGNKSHEIVQQGLRILHNLDHRGA VGADPLMGDGAGILLQIPDQFFREEMAKQGINLPPAGEYGVGMIFLPKEHASRLACEQEL ERTVRLEGQVVLGWRDVPVDKTMPMSPTVQKTEPVIRQIFIGRGRDIMTTDALERKLYVI RKTASHAIQALKLKHGKEYFVPSMSARTIVYKGLLLCEQVGRYYQDLADPRTVSALALVH QRFSTNTFPAWELAHPYRMVAHNGEINTVKGNVNWINARTGGISSPVLGDDLPKLWPLIY PGQSDTASFDNCLELLTMAGYPLAQAMMMMIPEAWEQHTLMDDNRRAFYEYHAAMMEPWD GPAAICFTDGRQIGATLDRNGLRPARYYVTDDDMVVMASEAGVLPIPESRIVKKWRLQPG KMFLIDMEQGRIIDDKELKDNLANAKPYKSWIDAVRIKLDELEAKPEDVAAETKPAAKLL DRQQAFAYTQEDVKFLMAPMAANGEEAAGSMGNDSPLAVLSSKNKTLYNYFKQLFAQVTN PPIDPIRENVVMSLVSFIGPKPNLLELNNINPPMRLEVSQPVLDFKDMAKIRNIEHYTGG KFMAYELNICYPVAWGKEGIEARLASLCAEAVDAVKSGYNILIVSDRAVDEKQAAIPALL ATSAIHHHLVEKGLRTSTGLVVETGSAREVHHFALLAGYGAEAVHPYLAMETLAELAPGL GLTAEKAIYNFTKAIGKGLHKVMSKMGISTYMSYTGAQIFEAIGLSRELVDKYFQGTASN VGGIGIFEVAEEALRLHRDAFGDAPVLANMLDAGGEYAYRVRGEEHMWTPDSIAKLQHAT RANSYQTYKEYANLINDQSKRHMTLRGLFEFKVDPARAIPLEEVEPAKEIVKRFATGAMS LGSISTEAHTTLAVAMNRIGGKSNTGEGGEDERRYRNELRGIPIKQGTKLSDVIGREVVE RDLELQEGDSLRSKIKQVASGRFGVTAEYLASADQIQIKMAQGAKPGEGGQLPGHKVTDY IGKLRYAVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPRSDVSVKLVSEVGVGTVAAG VAKAKADHVVIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLMLNGLRNRIRVQADGQ MKTGRDVAIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRKKFSGKP EHVVNYFFFVAEEVREIMAQLGIRTFNELIGRADLLDTKAGIEHWKARGLDFARIFYQPA KKEGEPCYQVDVQDHGLDRALDHQLIEKSKAALEKGERVSFIQPVRNVNRTVGAMLSGEV AKRYGHEGLPDDSIHVQMQGTAGQSFGAFLAHGITLDLVGDGNDYVGKGLSGGRVIVRPP HEFRGEPTSNIIVGNTVLYGALAGEAFFNGVAGERFAVRNSGATTVVEGTGDHGCEYMTG GTVVVLGATGRNFAAGMSGGVAYVYDEDGLFDKRCNTAQVALESVLSAADQEKAHTPSTW HKVNGERVLDEQLLKALVEKHFRYTGSERAKELLADWNNARRRFVKVFPTEYKRALAEMY EREQEAGREQIAA