Protein Info for ABZR87_RS02145 in Ralstonia sp. UNC404CL21Col

Annotation: aminodeoxychorismate/anthranilate synthase component II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00566: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
  • amino acids 1 to 186 (186 residues), 272.8 bits, E=8.1e-86
• PF00117: GATase
  • amino acids 3 to 186 (184 residues), 216.3 bits, E=3e-68
• PF07722: Peptidase_C26
  • amino acids 70 to 170 (101 residues), 46.2 bits, E=4.8e-16

Best Hits

Swiss-Prot: 67% identical to TRPG_ACIAD: Anthranilate synthase component 2 (trpG) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01658, anthranilate synthase component II [EC: 4.1.3.27] (inferred from 100% identity to rpf:Rpic12D_2759)

MetaCyc: 66% identical to anthranilate synthase beta subunit (Pseudomonas aeruginosa PAO1)
Anthranilate synthase. [EC: 4.1.3.27]

Predicted SEED Role

"Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85)" (EC 2.6.1.85, EC 4.1.3.27)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• superpathway of chorismate metabolism (44/59 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• 4-hydroxy-2(1H)-quinolone biosynthesis (3/5 steps found)
• acridone alkaloid biosynthesis (1/4 steps found)
• superpathway of quinolone and alkylquinolone biosynthesis (3/10 steps found)
• superpathway of candicidin biosynthesis (3/11 steps found)
• chloramphenicol biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Folate biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.85, 4.1.3.27

Use Curated BLAST to search for 2.6.1.85 or 4.1.3.27

Protein Sequence (189 amino acids)

>ABZR87_RS02145 aminodeoxychorismate/anthranilate synthase component II (Ralstonia sp. UNC404CL21Col)
MLLMLDNYDSFTYNIVQYFGELGQDVHTVRNDEITIEEIEKLNPERICLSPGPCTPTEAG
ILVPALKHFAGRVPILGVCLGHQAIADAFGGRVIRAQKVMHGKVSTIENTGVGVFANLPR
HFKVTRYHSLAIERETLPDCLEITAWTEDGEIMGVRHKTLPVEGVQFHPESILSEHGHAL
LGNFLKERF