Protein Info for ABZR87_RS02020 in Ralstonia sp. UNC404CL21Col

Annotation: long-chain fatty acid--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 30 to 429 (400 residues), 322.5 bits, E=3.3e-100
• PF13193: AMP-binding_C
  • amino acids 480 to 554 (75 residues), 64 bits, E=2e-21

Best Hits

Swiss-Prot: 56% identical to LCFA_YERPE: Long-chain-fatty-acid--CoA ligase (fadD) from Yersinia pestis

KEGG orthology group: K01897, long-chain acyl-CoA synthetase [EC: 6.2.1.3] (inferred from 99% identity to rpf:Rpic12D_2739)

MetaCyc: 54% identical to medium-chain-fatty-acid--CoA ligase (Pseudomonas aeruginosa PAO1)
Butyrate--CoA ligase. [EC: 6.2.1.2, 6.2.1.3]

Predicted SEED Role

"Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 6.2.1.16)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• oleate β-oxidation (32/35 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• fatty acid salvage (6/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• long-chain fatty acid activation (1/1 steps found)
• phytol degradation (3/4 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• valproate β-oxidation (6/9 steps found)
• octane oxidation (3/5 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• phosphatidylcholine acyl editing (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• capsaicin biosynthesis (3/7 steps found)
• stearate biosynthesis I (animals) (2/6 steps found)
• 2-deoxy-D-ribose degradation II (3/8 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• 4-oxopentanoate degradation (3/9 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• sporopollenin precursors biosynthesis (8/18 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• cutin biosynthesis (3/16 steps found)
• suberin monomers biosynthesis (5/20 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16, 6.2.1.3

Use Curated BLAST to search for 6.2.1.16 or 6.2.1.2 or 6.2.1.3

Protein Sequence (569 amino acids)

>ABZR87_RS02020 long-chain fatty acid--CoA ligase (Ralstonia sp. UNC404CL21Col)
MDKPWLKQYPAGVPAEIDASQYRSLAHLLEDSFQKNRNRRAFECMGKVLTYGELDTLSRQ
LAAWLQSRGLAPGARVALMMPNVLQYPVALAAVLRAGYVVVNVNPLYTPRELEHQLKDSG
AEAIIILENFATTLQQVLPNTPVKHIVVASMGDMLGGLKGMLVNFVVRSVKKMVPAWELP
NCIRFNTALEEGAKQTLKPTSVGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQSEAW
MNPALTKPGRDGRMLAADEEIVTITALPLYHIFALTVCCLLSMRKGGLAVLIPNPRDIPG
FIKVLKQYRFHMFPAVNTLYNALLNHPEFKDVDCSNLRVANGGGMAVQEAVAKKWLEVTG
CPIIEGYGLSETSPSAICNPTDTDKFSGTVGLPIPGTEIAIRDDEGRDLPIGQAGEICIR
GPQVMAGYWKRPDETAKVMYADGFFKTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNE
IEGVVAMCPGVLEVAAVGVPDVHSGEVVKLFVVRRDPSLTEQKLQEFCKDQLTGYKRPKF
IEFRNELPKTNVGKILRRELRDEAMKKRA