Protein Info for ABZR87_RS01855 in Ralstonia sp. UNC404CL21Col

Annotation: A24 family peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 transmembrane" amino acids 15 to 37 (23 residues), see Phobius details amino acids 114 to 130 (17 residues), see Phobius details amino acids 132 to 151 (20 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 185 to 205 (21 residues), see Phobius details amino acids 223 to 251 (29 residues), see Phobius details amino acids 263 to 290 (28 residues), see Phobius details PF06750: A24_N_bact" amino acids 24 to 129 (106 residues), 122.8 bits, see alignment E=4.9e-40 PF01478: Peptidase_A24" amino acids 140 to 248 (109 residues), 103.7 bits, see alignment E=7e-34

Best Hits

Swiss-Prot: 58% identical to LEP4_PSEAE: Type 4 prepilin-like proteins leader peptide-processing enzyme (pilD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02654, leader peptidase (prepilin peptidase) / N-methyltransferase [EC: 2.1.1.- 3.4.23.43] (inferred from 96% identity to rpf:Rpic12D_2707)

MetaCyc: 58% identical to prepilin leader peptidase/N-methyltransferase (Pseudomonas aeruginosa)
Prepilin peptidase. [EC: 3.4.23.43]; 2.1.1.- [EC: 3.4.23.43]

Predicted SEED Role

"Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-)" in subsystem Type IV pilus (EC 2.1.1.-, EC 3.4.23.43)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-, 3.4.23.43

Use Curated BLAST to search for 2.1.1.- or 3.4.23.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (293 amino acids)

>ABZR87_RS01855 A24 family peptidase (Ralstonia sp. UNC404CL21Col)
MPELFVIPTLAQLPAWFVIAIGALVGLLVGSFLNVVIHRLPRMIEREEANYIAELREEPL
PHPDAYNLVVPRSACPSCGHQIKAIENIPVLSWLALRGRCSACKTPISWRYPAVELVTGL
LTAACLWHFGPTWVAVASAVLLWFLIAGTMIDADTQLLPDAVTQPLLWLGLGVNLFGMFA
HLHDAVIGAMAGYLFLWSIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGLVRIARGATSESPFSFGPFIAGAGVIALLAGPQLLMLAGLGPLLAP