Protein Info for ABZR87_RS01410 in Ralstonia sp. UNC404CL21Col

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 877 TIGR00396: leucine--tRNA ligase" amino acids 6 to 876 (871 residues), 1076.4 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 177 (164 residues), 83 bits, see alignment E=4.9e-27 amino acids 433 to 588 (156 residues), 32.6 bits, see alignment E=9.7e-12 amino acids 635 to 675 (41 residues), 26.2 bits, see alignment 8.8e-10 PF09334: tRNA-synt_1g" amino acids 44 to 177 (134 residues), 65 bits, see alignment E=1.4e-21 PF13603: tRNA-synt_1_2" amino acids 226 to 419 (194 residues), 225.6 bits, see alignment E=9.3e-71 PF08264: Anticodon_1" amino acids 717 to 839 (123 residues), 57.3 bits, see alignment E=4.2e-19

Best Hits

Swiss-Prot: 76% identical to SYL_BURCJ: Leucine--tRNA ligase (leuS) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 76% identity to bcj:BCAL3373)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (877 amino acids)

>ABZR87_RS01410 leucine--tRNA ligase (Ralstonia sp. UNC404CL21Col)
MQDKYSPSDVEQQAQQHWQALDAYRVTEHARAADGSDKPKFYACSMLPYPSGKLHMGHVR
NYTINDVMTRQLRMKGYNVLMPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQS
MGLAIDWSREVATCSPDYYRWNQWLFLKMLEKGIAYRKTGTVNWDPVDQTVLANEQVIDG
RGWRSGAVVEKREIPMYYLGITKYAQELLSDLDPLGWPERVKLMQQNWIGKSEGVRFAFP
HNIAGDDGKLINDGKLYVFTTRADTIMGVTFCAVAAEHPIAAHAAQSNPALAAFIEECKH
GSVMEADMATMEKKGMPTGLTVTHPLTGESVPVWVGNYVLMTYGDGAVMGVPAHDERDFA
FANKYHLPIKQVIDVKGQPYDTTTWADWYGDKEHGVLFHSGKYDGLNYQQAVDAVAADLA
AQGLGEKKTTWRLRDWGISRQRYWGTPIPLIHCESCGVVPVPEQDLPVRLPEDLVPDGTG
NPLAKDPRFLNCTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDGETMVDARNDYWMPM
DQYIGGIEHAILHLLYARFWTKVMRDLGLVKFDEPFTNLLTQGMVLNETYYREDAAGKKQ
WINPADVDVQTDERGRPVGATLKADGQPVVIGGVEKMSKSKNNGIDPQALIDQYGADTAR
LFTMFAAPPEQQLEWNDAGVEGASRFLRRLWNFGVAHADAIRAGHGNGVVAGATDADRAL
RRELYTVLKQANYDYERLQYNTVVSATMKMLNALEGAKDAGADARREGLGLLLRVLYPVV
PHITHVLWTELGYAGAYGDLLDAPWPQVDEGALVQSEIELVLQVNGKVRGSIVVPADADR
AAIEAIAAKDEAVQKFAEGKPPKKIIVVPGRLVNVVA