Protein Info for ABZR87_RS01135 in Ralstonia sp. UNC404CL21Col

Annotation: FAD-linked oxidase C-terminal domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01565: FAD_binding_4
  • amino acids 56 to 194 (139 residues), 149.2 bits, E=6.5e-48
• PF02913: FAD-oxidase_C
  • amino acids 230 to 470 (241 residues), 220.1 bits, E=4.1e-69

Best Hits

Swiss-Prot: 60% identical to GLCD_ECOLI: Glycolate oxidase subunit GlcD (glcD) from Escherichia coli (strain K12)

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 99% identity to rpf:Rpic12D_2496)

MetaCyc: 60% identical to glycolate dehydrogenase, putative FAD-linked subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

• glycolate and glyoxylate degradation II (2/2 steps found)
• glycolate and glyoxylate degradation I (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• glycolate and glyoxylate degradation III (1/3 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• photorespiration II (5/10 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14

Use Curated BLAST to search for 1.1.3.15 or 1.1.99.14

Protein Sequence (499 amino acids)

>ABZR87_RS01135 FAD-linked oxidase C-terminal domain-containing protein (Ralstonia sp. UNC404CL21Col)
MNAPSSSSATARDLTALQAELTAALSQIVSDDALLWEREDTTPYECDGLAAYRQVPLAVV
LPDTEAQVVEILRVCHRMGVPVVPRGAGTSLSGGAMPTPGGLVLSLAKFKRILKVDPYTR
TAVVQPGVRNLAISEAANPHGLYYAPDPSSQIACTIGGNVNENSGGVHCLKYGLTVHNVL
RVRAVMMDGEVVEFGSEAPDAPGLDLLATVIGSEGMLAVVTEVTVRLIPKPQLAQVIMAS
FDDVESGGNAVADVIAAGIIPAGLEMMDKPATAAVEEFVCAGYDLDAAAILLCESDGTPE
EVAEEIARMSEVLRASGATRIQVSQSEAERLKFWSGRKNAFPAAGRISPDYYCMDGTIPR
KHIGTLLRRIQAMEQKYALRCINVFHAGDGNMHPLILFNGEDQDEWHRAELFGSDILETC
VELGGTVTGEHGVGVEKLSSMCVQFSTEERDAFLRVKAAFDPARLLNPDKAIPTLARCAE
YGKLHVRNGLLPHPDLPRF