Protein Info for ABZR87_RS00835 in Ralstonia sp. UNC404CL21Col

Annotation: argininosuccinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00764: Arginosuc_synth
  • amino acids 13 to 161 (149 residues), 69.5 bits, E=3.7e-23
• TIGR00032: argininosuccinate synthase
  • amino acids 13 to 420 (408 residues), 523.8 bits, E=1.8e-161
• PF20979: Arginosuc_syn_C
  • amino acids 191 to 404 (214 residues), 112.4 bits, E=2.4e-36

Best Hits

Swiss-Prot: 100% identical to ASSY_RALPJ: Argininosuccinate synthase (argG) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01940, argininosuccinate synthase [EC: 6.3.4.5] (inferred from 100% identity to rpi:Rpic_2829)

Predicted SEED Role

"Argininosuccinate synthase (EC 6.3.4.5)" in subsystem Arginine Biosynthesis extended (EC 6.3.4.5)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• urea cycle (4/5 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.5

Protein Sequence (445 amino acids)

>ABZR87_RS00835 argininosuccinate synthase (Ralstonia sp. UNC404CL21Col)
METILQHVPVGQKVGIAFSGGLDTSAALRWMKNKGALPYAYTANLGQPDEEDYDAIPRKA
MEYGAEKARLIDCRPQLANEGIAAIQSGAFHISTGGITYFNTTPLGRAVTGTMLVAAMKE
DDVNIWGDGSTFKGNDIERFYRYGLLTNPALKIYKPWLDQAFIDELGGRAEMSAFMTKEG
FGYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGIRIVNPIMGVAFWKPEVEVKAEEVSVT
FDEGRPVAVNGREIADPVEMFLELNRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
HIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDPQAIMLRETAQRWVARAVTGTV
TLELRRGNDYSILNTESPNLTYAPERLSMEKVEDAPFSPADRIGQLTMRNLDLMDTRDKL
AIYSKAGLLSLGTANALPQLDGGKK