Protein Info for ABZR87_RS00420 in Ralstonia sp. UNC404CL21Col

Annotation: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00521: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase
  • amino acids 3 to 392 (390 residues), 419.9 bits, E=5.5e-130
• PF02441: Flavoprotein
  • amino acids 6 to 177 (172 residues), 120.8 bits, E=4.3e-39
• PF04127: DFP
  • amino acids 185 to 367 (183 residues), 213.7 bits, E=2e-67

Best Hits

Swiss-Prot: 52% identical to COABC_VIBVU: Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) from Vibrio vulnificus (strain CMCP6)

KEGG orthology group: K13038, phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase [EC: 4.1.1.36 6.3.2.5] (inferred from 98% identity to rpf:Rpic12D_2340)

MetaCyc: 47% identical to fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase (Escherichia coli K-12 substr. MG1655)
Phosphopantothenate--cysteine ligase. [EC: 6.3.2.5]; Phosphopantothenoylcysteine decarboxylase. [EC: 6.3.2.5, 4.1.1.36]

Predicted SEED Role

"Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)" in subsystem Coenzyme A Biosynthesis (EC 4.1.1.36, EC 6.3.2.5)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis I (bacteria) (9/9 steps found)
• superpathway of coenzyme A biosynthesis III (mammals) (5/5 steps found)
• coenzyme A biosynthesis I (bacteria) (4/4 steps found)
• coenzyme A biosynthesis II (eukaryotic) (4/4 steps found)
• coenzyme A biosynthesis III (archaea) (3/4 steps found)
• taurine biosynthesis II (1/4 steps found)

KEGG Metabolic Maps

• Pantothenate and CoA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.36 or 6.3.2.5

Protein Sequence (401 amino acids)

>ABZR87_RS00420 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC (Ralstonia sp. UNC404CL21Col)
MELQGKHFVLGLTGGIACYKSAELVRLLTKAGATVQVVMTEAATHFITPVTMQALSGRPV
FTSQWDGRIGNNMPHIDLSREADAIVIAPASTDFLAKLAHGMADDLLSTLCIARDCPLLV
APAMNRQMWAAPATQRNAAQLRADDVTLLGPDAGDQACGEVGDGRMLEPEDIVEQLIGFF
QPKLLRGHRLLLTAGPTFEPIDPVRGITNLSSGKMGFALARAASQAGAEVTLVAGPCHQD
TPAGVLRIDVQTAQQMHDAVMRELDTTRNDIFIAVAAVADWRVAQAAEQKLKKQSDSDVP
TLTFTQNPDILAAVARRANAPFCVGFAAETERLEEFGEAKRQKKGIPLLVGNLGHQTFGR
DDNEVALFDAKGITRLPRADKLTLARQIVAAIADRVHGARS