Protein Info for ABZR87_RS00395 in Ralstonia sp. UNC404CL21Col

Annotation: bifunctional riboflavin kinase/FAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 PF06574: FAD_syn" amino acids 11 to 165 (155 residues), 190.8 bits, see alignment E=2.1e-60 TIGR00125: cytidyltransferase-like domain" amino acids 17 to 75 (59 residues), 30.3 bits, see alignment E=3.7e-11 TIGR00083: riboflavin biosynthesis protein RibF" amino acids 17 to 306 (290 residues), 302.7 bits, see alignment E=2.8e-94 PF01467: CTP_transf_like" amino acids 18 to 169 (152 residues), 23.8 bits, see alignment E=6.9e-09 PF01687: Flavokinase" amino acids 183 to 306 (124 residues), 129.8 bits, see alignment E=1e-41

Best Hits

Swiss-Prot: 50% identical to RIBF_ECOL6: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 97% identity to rpf:Rpic12D_2336)

MetaCyc: 50% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (332 amino acids)

>ABZR87_RS00395 bifunctional riboflavin kinase/FAD synthetase (Ralstonia sp. UNC404CL21Col)
MKVFRGLPNAESRAPCALTIGNFDGVHRGHQSLLARARAAADARGLPLTVMTFEPHPREF
FMPDRAPTRIALLRDKLESLRRQGVDRVVVEHFNAHFAGQTPDEFVRNVLVDGLHARWLL
VGDDFRFGAKRAGDFTYLQAAGAQFGFEVEQMGSVSESGIRISSSAVREALSAGDLEHAR
RLLGHGYAISGHVVHGQKLGRSLGFPTLNLRISHRKPAVAGIFVVQVHGLAEKPLPAVAS
IGVRPTVDDSGRVLLEVHVFDYNASVYGKLVRVEFMKKLRDEARYDSLDALKDAIAQDCV
HARQFFGLPVPPAGESPMLRRDFATSATDRIQ