Protein Info for ABZR87_RS00200 in Ralstonia sp. UNC404CL21Col

Annotation: phosphoadenylyl-sulfate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01507: PAPS_reduct
  • amino acids 59 to 234 (176 residues), 142.2 bits, E=9e-46
• TIGR02055: adenylylsulfate reductase, thioredoxin dependent
  • amino acids 61 to 258 (198 residues), 267.6 bits, E=3.3e-84

Best Hits

Swiss-Prot: 57% identical to CYSH_NEIMB: Phosphoadenosine phosphosulfate reductase (cysH1) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K00390, phosphoadenosine phosphosulfate reductase [EC: 1.8.4.8] (inferred from 98% identity to rpf:Rpic12D_2227)

MetaCyc: 53% identical to assimilatory adenylyl-sulfate reductase (Allochromatium vinosum)
Adenylyl-sulfate reductase (glutathione). [EC: 1.8.4.9]

Predicted SEED Role

"Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10)" in subsystem Cysteine Biosynthesis (EC 1.8.4.10, EC 1.8.4.8)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• selenate reduction (2/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.4.10 or 1.8.4.8 or 1.8.4.9

Protein Sequence (275 amino acids)

>ABZR87_RS00200 phosphoadenylyl-sulfate reductase (Ralstonia sp. UNC404CL21Col)
MSDVQDVSVSEVPVSAIGRPVLWTRPAYTGTAEALAAKEAALFERLAEIAAKHGVAKFAT
SLAAEDMVVTDAILRSPEAVRAHLPIFTLQTGRLHAETLEMLDRVRAHYGYAIEQYAPDA
RAVEAYVRDHGLNAFYDSIELRKACCNIRKVVPLNRALKDADAWLTGQRREQAVTRADLP
FAEDDEARGIAKYNPLFDWSEAEVWAYLEQHNVPTNALHDKGYPSIGCEPCTRAVRAGED
VRAGRWWWESRDSKECGLHAANAVTSSATHPSQQN