Protein Info for ABZR86_RS19560 in Dyella japonica UNC79MFTsu3.2

Updated annotation (from data): aspartate kinase / diaminopimelate decarboxylase (EC 4.1.1.20; EC 2.7.2.4)
Rationale: Important for fitness in most defined media. Contains TIGR00657 and TIGR01048.
Original annotation: bifunctional aspartate kinase/diaminopimelate decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00657: aspartate kinase
  • amino acids 14 to 465 (452 residues), 318.1 bits, E=1.1e-98
• PF00696: AA_kinase
  • amino acids 15 to 295 (281 residues), 148 bits, E=6e-47
• TIGR01048: diaminopimelate decarboxylase
  • amino acids 514 to 869 (356 residues), 315.2 bits, E=5.2e-98
• PF02784: Orn_Arg_deC_N
  • amino acids 526 to 758 (233 residues), 128.8 bits, E=3.6e-41
• PF00278: Orn_DAP_Arg_deC
  • amino acids 733 to 848 (116 residues), 82.5 bits, E=3.2e-27

Best Hits

KEGG orthology group: K12526, bifunctional diaminopimelate decarboxylase / aspartate kinase [EC: 2.7.2.4 4.1.1.20] (inferred from 56% identity to xcv:XCV3056)

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Diaminopimelate decarboxylase (EC 4.1.1.20)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis (EC 2.7.2.4, EC 4.1.1.20)

MetaCyc Pathways

• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• aspartate superpathway (21/25 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• ectoine biosynthesis (2/5 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• norspermidine biosynthesis (2/6 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• superpathway of polyamine biosynthesis III (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.7.2.4, 4.1.1.20

Use Curated BLAST to search for 2.7.2.4 or 4.1.1.20

Sequence Analysis Tools

See A0A1I2JKT2 at UniProt or InterPro

Protein Sequence (870 amino acids)

>ABZR86_RS19560 aspartate kinase / diaminopimelate decarboxylase (EC 4.1.1.20; EC 2.7.2.4) (Dyella japonica UNC79MFTsu3.2)
MNPQSPASLPADASWIVMKFGGTSVATLPRWQNIRELVASRRAEGARVLVVVSALTGITD
ALKQMCAQEDKGKRVEAARAIAQRHYDLLDHMQLAVPATLAERLSELAMLAEDGPGAMGE
LAWAALVQAHGELMSSALGAAFLSHAGLPTEWLDARDCLAAVALPNQNERTKLLSAMVEA
RPDPALNARLAELGEVFITQGFIAREAQGRTVLLGRGGSDTSASYFGALLKAQRVEIWTD
VAGMFTANPRQVPGARLLQRLDYEEAQEIASTGAKVLHPRCLSPLREPRVPMLIKDTNRP
ELEGTVIGPEVREHAPSVKAISARKGITLVSMESVGMWQQVGFLADVFAQFKQHGLSVDL
IGSAETNVTVSLDPTENLLDSDAIAALASDLAKVCRVKVIAPCAAITLVGRGMRSMLHTL
SGVLAEFGQIRVHLISQSSNNLNLTFVVDENVVDDLLPHLHELLIAAGALRTDDSALFGP
SWQALYGSGEVAAPAASWWRTAERERLLRLGAERTPRYVYHLPTVRQQARELKSLGAVDR
LHYAVKANTHPAILKALAEEGFGFECVSPGELKAVLAAVPESAPLLFTPNFAPRADYEWA
LTTRATISLDALYQLENWGELFRGREIVLRVDLGRGLGHHEKVRTGGSGSKFGLPVELVD
SFLRLADAHGVIVRGLHAHLGSGILDEGHWGEVYGQLASLAERIGSVAFLDIGGGLGVPS
HPGEARLDIAALDRVLRSVKAAYPHYQLWMEPGRYLVADAGVLLARVTQQKGKGSWRYLG
VDTGMNSLIRPALYEAWHEIVNLTRLDEPATGLFQVVGPICESGDVLGTDRRLPEAQEGD
VVLVAQAGAYGKVMSSPYNMRDEAEEVILD