Protein Info for ABZR86_RS18715 in Dyella japonica UNC79MFTsu3.2

Annotation: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 2 to 469 (468 residues), 687.3 bits, see alignment E=5.3e-211 PF00483: NTP_transferase" amino acids 4 to 283 (280 residues), 186.1 bits, see alignment E=1.3e-58 PF01050: MannoseP_isomer" amino acids 315 to 465 (151 residues), 244.9 bits, see alignment E=4.5e-77 PF07883: Cupin_2" amino acids 382 to 449 (68 residues), 48.1 bits, see alignment E=1.2e-16

Best Hits

Swiss-Prot: 56% identical to MANC1_ECO57: Mannose-1-phosphate guanylyltransferase 1 (manC1) from Escherichia coli O157:H7

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 58% identity to tbd:Tbd_1239)

MetaCyc: 56% identical to mannose-1-phosphate guanylyltransferase (Escherichia coli K-12 substr. MG1655)
Mannose-1-phosphate guanylyltransferase. [EC: 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.13, 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2G9P1 at UniProt or InterPro

Protein Sequence (470 amino acids)

>ABZR86_RS18715 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Dyella japonica UNC79MFTsu3.2)
MLIPLVLSGGSGTRLWPVSRKNLPKQFLSLMGQGTLFQQTVARTRLLPDVASPIVVASED
HRFLVAEQLLESGIQDATIVLEPLPRNTAPAIALGALQAVGQDPEALLLVLPADHLIGDE
DSFRDAVEQALPAARDNWLVTFGIRPDRPETGFGYIRRGDAIGGHAYKVAEFVEKPALET
AQGYIEHGGYDWNSGMFLFKAARFLDELAAHAPAMLQAVRAAHASAKADLDFVRVDADAF
AQVPENSIDYAVMEKTRHAAVIPVSCAWSDIGSWSALWLAGVRDAQDNLREGDTIAIKTH
RSLLRSHDRHLLATVGVDDLIVVTTPDATLVAHRDAAQDVKRVVDELKAAGRTEHSLHRV
VRRPWGSYDSLESADRFQVKRIVVKPGAALSLQKHHHRAEHWIVVSGTAEVTCDDKVFLL
GENQSTYIPLGSVHRLRNPGKVPLEIIEVQSGSYLGEDDIVRLEDVYGRS