Protein Info for ABZR86_RS18200 in Dyella japonica UNC79MFTsu3.2

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1031 TIGR00594: DNA polymerase III, alpha subunit" amino acids 5 to 976 (972 residues), 547.4 bits, see alignment E=3.7e-168 PF02811: PHP" amino acids 6 to 108 (103 residues), 67.3 bits, see alignment E=5.4e-22 PF07733: DNA_pol3_alpha" amino acids 268 to 529 (262 residues), 275.5 bits, see alignment E=1.3e-85 PF17657: DNA_pol3_finger" amino acids 532 to 698 (167 residues), 201.4 bits, see alignment E=1.6e-63 PF14579: HHH_6" amino acids 772 to 862 (91 residues), 73.8 bits, see alignment E=2.6e-24 PF01336: tRNA_anti-codon" amino acids 941 to 1010 (70 residues), 32 bits, see alignment 2.3e-11

Best Hits

Swiss-Prot: 63% identical to DNAE2_PSE14: Error-prone DNA polymerase (dnaE2) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 64% identity to pmk:MDS_2523)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2J6S0 at UniProt or InterPro

Protein Sequence (1031 amino acids)

>ABZR86_RS18200 error-prone DNA polymerase (Dyella japonica UNC79MFTsu3.2)
MTDYAELHCLSNFSFQRGASSAAELFERAKACGYRALAITDECSLAGIVRAYEASLETGL
ELIVGAEFKLDDGLRFVLLCENREGYTSLCGLITRGRRASAKGTYHLTRADLADGLPGTL
ALWLPETAPNADDGRWLAERFAGHLWLAVELHRGPDDAARLRQLDMLASELGIPAVAAGD
VHMHVRGRRALQDTLTAIRHGLTVAQAGAVLFKNGERHLRTREALAEIYPASLLEETARV
AARCSFNLDQLHYRYPAELVPAGHTAASWLRKLTEEGIERRWPDGVSAKNRKQIETELAL
IRELEYEHYFLTVEDIVRYARGIDILCQGRGSAANSVVCYALGVTSVSPDEAQLLTERFM
SASRKDHKYNRPEPPDIDIDFEHERREEVIQYIYRKYGRERTALAATVISYRGRSAARDV
GKALGLSTDQVAALSDVFAWRNGGDLNEHLRQAGLDPASPLLERVILLTEELRGFPRHLS
QHVGGFVISDAPLSQLVPVENAAMPERTIIQWDKDDLDRMNLLKVDCLALGMLTCVRKCF
DLMKQHEGVSHTLASIPIGDEPTYAMVQQADTIGVFQIESRAQMSMLPRLRPENYYDLVI
QVAIVRPGPIQGDMVHPYLRRRRGEEEVTYPSDALKEVLERTLGVPLFQEQVMRIAMVAA
GYTGPQADELRRAMAAWKRHGGMEKHRDQLFSGMKERGYSEAFAAQIFEQIKGFGSYGFP
ESHAAGFAKLAYASAWLKCHHPAAFACALLNAQPMGFYSASQIVQDARRHEVAVLPVDVR
YSDWDCTLERDTRSKGGFALRLGLRQVRGCSETMARRIEQARAQAPFADIVDLCTRASLD
RRHQDLLADAGALRGLAGHRHRAKWAVSGVEAQLPLFGRSSPTEAAVALPPPTSAENLLA
DYAHTGLSLGPHPVRLLRARLRAQRFLDSKQIGGLPHQAPVRMAGLVVQRQQPQTASGVT
FITLEDEHGPINVVVWRQVAERQHRPYLEARLLGVEGRWETVDGVCHLIARRLLDLSPLL
GELDVRSRDFH