Protein Info for ABZR86_RS17230 in Dyella japonica UNC79MFTsu3.2

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 22 to 99 (78 residues), 106.5 bits, E=5e-35
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 139 to 591 (453 residues), 720.7 bits, E=2.6e-221
• PF01964: ThiC_Rad_SAM
  • amino acids 140 to 587 (448 residues), 625.4 bits, E=4.6e-192

Best Hits

Swiss-Prot: 83% identical to THIC_XANOP: Phosphomethylpyrimidine synthase (thiC) from Xanthomonas oryzae pv. oryzae (strain PXO99A)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 84% identity to psu:Psesu_0451)

MetaCyc: 70% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See A0A1I2HT10 at UniProt or InterPro

Protein Sequence (626 amino acids)

>ABZR86_RS17230 phosphomethylpyrimidine synthase ThiC (Dyella japonica UNC79MFTsu3.2)
MNAQPNDLARAAQELSEAVTRPIPGSRKIYVQGSRADLRVPLREICQAKTPTLFGGEDNP
PVTVYDTSGPYTDPGYQVDLAAGLPALRAAWIEERGDVERLTGLSSDFGRQRADDARLDP
IRFRHHLRQPLRAKAGANVTQMHYARRGIVTPEMEYIAIRENQKLDALAESALRNQHPGQ
SFGASLPRLITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVSSG
IAEEVEKLVWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVGGVTE
ELTWELFRDTLVEQAEQGVDYFTIHAGVLLRFVPLTARRVTGIVSRGGSIMAKWCLAHHK
ENFLYTHFEEICEIMKAYDVSFSLGDGLRPGCVADANDAAQFGELDTLGELTQIAWKHDV
QTMIEGPGHVPMQLIKENMERQLEKCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWF
GTAMLCYVTPKEHLGLPNKQDVRDGIIAYKIAAHAADLAKGHPGAQVRDNALSKARFEFR
WDDQFNLGLDPEKAKEFHDETLPKDAHKSAHFCSMCGPKFCSMKITQDVRDYAAEHGVDE
TSALDEGMAEKAAEFREQGSEVYHRA