Protein Info for ABZR86_RS15970 in Dyella japonica UNC79MFTsu3.2

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 TIGR00973: 2-isopropylmalate synthase" amino acids 21 to 514 (494 residues), 653 bits, see alignment E=1.2e-200 PF00682: HMGL-like" amino acids 21 to 289 (269 residues), 313.8 bits, see alignment E=1.1e-97 PF08502: LeuA_dimer" amino acids 386 to 516 (131 residues), 100.8 bits, see alignment E=5.7e-33

Best Hits

Swiss-Prot: 53% identical to LEU1_RHOBA: 2-isopropylmalate synthase (leuA) from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 54% identity to sur:STAUR_3608)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2JHE1 at UniProt or InterPro

Protein Sequence (535 amino acids)

>ABZR86_RS15970 2-isopropylmalate synthase (Dyella japonica UNC79MFTsu3.2)
MNHETQHSNDSSEDGVVADDRVRIFDTTLRDGEQAPGFGMDRRAKLRMAHALEALGVDVM
EAGFPQASPDDFAAVADIAKAVRHSTVCALARCQAADIDTAGRALEAAQHSRIHVFLSTS
PLHREHKLGMSKQQVIDTAIAAVERARALCHEVEFSAEDAMRTEPDYLAEVFSAAIAAGA
TTVNAPDTVGYVTPAEIAERFAYLRKHVKGAERVVFSSHCHDDLGMAVANSLAAVSAGAR
QIECTINGIGERAGNASLEEVVMALRVRGPYFGVDSRIDARRLVQTSRLLTQLTGQAVPR
NKAIVGDNAFAHESGIHQHGMLKHRGTYEIMRPQDVGMGETKLVLGKHSGRHALRSRLQA
LGHTPEEAAMDDIFARFKALADKKREIHDEDLEALALGQDPDAAGPWRIVQLNSSSHLGG
SASASVRLAHDDGREIGEAAIGDGPVDAVLRAMERATGTDLELTQFQVRAVSEGGDAQGQ
AQLTARHAARNWRGNGVSTDIVEATALAALSIVNRIERQAAPSAAAQPQVQGATA