Protein Info for ABZR86_RS13865 in Dyella japonica UNC79MFTsu3.2

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 833 TIGR01051: DNA topoisomerase I" amino acids 5 to 622 (618 residues), 762.7 bits, see alignment E=2.3e-233 PF01751: Toprim" amino acids 5 to 117 (113 residues), 69.6 bits, see alignment E=3.3e-23 PF01131: Topoisom_bac" amino acids 133 to 566 (434 residues), 435.5 bits, see alignment E=3.3e-134 PF13368: Toprim_C_rpt" amino acids 598 to 657 (60 residues), 53.5 bits, see alignment 2.9e-18 amino acids 660 to 706 (47 residues), 50.7 bits, see alignment 2.2e-17 amino acids 719 to 763 (45 residues), 63.9 bits, see alignment 1.7e-21

Best Hits

Swiss-Prot: 65% identical to TOP1_XYLFT: DNA topoisomerase 1 (topA) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 71% identity to xal:XALc_2864)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2EFN8 at UniProt or InterPro

Protein Sequence (833 amino acids)

>ABZR86_RS13865 DNA topoisomerase I (Dyella japonica UNC79MFTsu3.2)
MAKNLLIVESPAKAKTINKYLGKDFQVLASYGHVRDLKPKEGAVDPDHGFAMAYEIIERN
QKHVDAIARAAQVADDIYLATDLDREGEAISWHISEILQQRGLTKGKQLHRVVFSEITPK
AIKAAVAQPRQLSHDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIV
EREEEIEAFVAREYWSVEAALRHPEGDFSARLTRLYGKKFEQFDLTNEADAMAARAALKE
AAHGRLTVSDVGSKERKRRPAPPFTTSTLQQEAARKLGFSTSRTMKVAQGLYEGVSLGSE
GNVGLITYMRTDSVALSEDAVGELRQLIARDYGNKALPDHPQVYKSKSKNAQEAHEAIRP
TSAMYTPREVASFLNDEQRKLYELIWKRTVACQMIHATLNTVSVDFALPHAQGGEAGFRS
TGTTVVDPGFLAVYEEGRDQKSAEDDDEGRRLPRLEKGEQVALHDILADQHFTEPPPRYS
EASLVKSLEEHGIGRPSTYASIIQVLLNREYVLLDSRRFKPTDVGRAVSKFLTQHFTRYV
DYDFTAKLEDELDAVSRGEEAWVPLMERFWQPFKQQVEEKTETVDRSEATGARELGNDPK
SGKPVSVRLGRYGPYAQIGDKDTDEKLQFASLRPGQSMHTITLEDALELFKLPRKLGQAE
NGDEVSVGVGRFGPFVKQGSTYASLKAEDDPYTIELPRALQIVQEKLELLANRVIQDFGN
GVQVLNGRFGPYITDGEKNARIPKDQDPKTLTEAQCVELLAAAPVKKGRGGARKAAAKKA
APAAKKAAAKKAPAEKPAAKKAAAKKTTAKKTATKKTAAKKATAKKPAGKAKA