Protein Info for ABZR86_RS13335 in Dyella japonica UNC79MFTsu3.2

Annotation: bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 PF01149: Fapy_DNA_glyco" amino acids 1 to 118 (118 residues), 121.7 bits, see alignment E=4e-39 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 273 (273 residues), 284.5 bits, see alignment E=4.2e-89 PF06831: H2TH" amino acids 134 to 224 (91 residues), 89.4 bits, see alignment E=1.9e-29 PF06827: zf-FPG_IleRS" amino acids 246 to 274 (29 residues), 43.4 bits, see alignment (E = 3.6e-15)

Best Hits

Swiss-Prot: 68% identical to FPG_STRMK: Formamidopyrimidine-DNA glycosylase (mutM) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 70% identity to xal:XALc_3160)

MetaCyc: 53% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2EPW1 at UniProt or InterPro

Protein Sequence (274 amino acids)

>ABZR86_RS13335 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase (Dyella japonica UNC79MFTsu3.2)
MPELPEVETTRRGIAPHLIGRRITSVVLRRPDLRWPIPPEVGALLPGQRIEEVERRAKYL
LLHTQVGSALLHLGMTGVLRVLPPDAPVGKHDHVDIALEATTVGARILRFTDARRFGCLL
WQPPGTTHELLENLGPEPLTDAFDGDLLWHLSRGRTAAVKLFLMDNAVVVGVGNIYASEA
LFAAGIDPRRAAGSVSRARYARLAAEVKRILAWAIERGGTTLRDFINPDGMPGYFVRELQ
VYGREGEPCRTCGTAIRQVVLGQRSTFWCPRCQK