Protein Info for ABZR86_RS12535 in Dyella japonica UNC79MFTsu3.2

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 PF17764: PriA_3primeBD" amino acids 6 to 101 (96 residues), 86 bits, see alignment E=3.8e-28 PF04851: ResIII" amino acids 194 to 324 (131 residues), 48.3 bits, see alignment E=3.2e-16 PF00270: DEAD" amino acids 214 to 360 (147 residues), 58.1 bits, see alignment E=2.8e-19 TIGR00595: primosomal protein N'" amino acids 216 to 724 (509 residues), 586.1 bits, see alignment E=2.9e-180 PF18319: PriA_CRR" amino acids 440 to 465 (26 residues), 33.7 bits, see alignment (E = 8.6e-12) PF00271: Helicase_C" amino acids 487 to 583 (97 residues), 28.9 bits, see alignment E=3.5e-10 PF18074: PriA_C" amino acids 627 to 723 (97 residues), 71.2 bits, see alignment E=3.2e-23

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 57% identity to psu:Psesu_2763)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2JCP1 at UniProt or InterPro

Protein Sequence (727 amino acids)

>ABZR86_RS12535 primosomal protein N' (Dyella japonica UNC79MFTsu3.2)
MPSVLRVALPVPLPTLFDYLPPAAGEARPGSRVLVPFGRGKAVGVVVETEVESALGGARL
KQALRLLDDTPLLDAELMKTLAWAADYWLGAPGEAYANALPLALREDKPLPDTHEEVWAL
TVAGRSALDAGSRKGGSRRLLETLAAAPLGAAELHERLPDWRDAARRLAAAGLIERVRRE
ADAAPQPVTAAPALSAEQHAAVDAVGAELGRFQPFLLDGVTGSGKTEVYLALIERVIAEG
KQALLLVPEIGLAPQTVRRVRERLGVPVEVLHSNLAEGDRARAWLRARDGEARVILGTRS
AVFTPLPRAGLIVVDEEHDGAYKQQEGFRYHARDLALLRGRALGVPVLLGSATPSLESLA
NVEAGRYRALHLRARPGAIRPPQVQIVDMRAQRLEHGLSPTLLAAVADAVARGEQALVFR
NRRGYAPVLLCHQCGWHAECPRCEHPLTLHAGRRQLICHHCDYHARLPEHCPSCDAPDLK
PQGQGTERLEEALAARFPDTPVLRIDRETTRRRDAFDELLERLRGEGPAILVGTQMLAKG
HDLPNLTLVAIVGVDEGLHSVDFRASERLAQLIVQVAGRAGRASKPGRVILQTHHPDHPL
LRQLLAQGYAAAAKELLAERRSVQLPPYMHQVLLRADAHQREHVEAFLHEAYGMLLPAAA
QAELAIAGPMPAPMPLRAGRHRGQLLVEAGSRAVLHAALRPWARGLSALPSARRVRWSLD
VDPVDLY