Protein Info for ABZR86_RS11350 in Dyella japonica UNC79MFTsu3.2

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 21 to 486 (466 residues), 541.2 bits, see alignment E=1.2e-166 PF01225: Mur_ligase" amino acids 23 to 98 (76 residues), 39.9 bits, see alignment E=6.6e-14 PF08245: Mur_ligase_M" amino acids 110 to 312 (203 residues), 177.7 bits, see alignment E=4.2e-56 PF02875: Mur_ligase_C" amino acids 333 to 419 (87 residues), 83.9 bits, see alignment E=1.3e-27

Best Hits

Swiss-Prot: 54% identical to MURE_XYLFT: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 54% identity to xfn:XfasM23_1973)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2HK39 at UniProt or InterPro

Protein Sequence (490 amino acids)

>ABZR86_RS11350 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Dyella japonica UNC79MFTsu3.2)
MNQRLDQLLHGIADAQGAGDIVVSGLALDSRRVRAGDAFFALRGTRGHGIEFAAGAVAQG
ARVVLAEAPATSATTLGVPVIWIDRLHTQVGEIAARYYGRPSQALQVVGVTGTNGKTSTV
QLIAQALHHLGHSPATIGTLGAGLHGQVEEGERTTPDAIHVQELLASFRTQGATHVAMEV
SSHALEQGRVGAVAFGTAVFTNLTRDHLDYHGSMEAYGDAKAKLFAWPTLTHAAINVDDA
FGRELLHRTPGHVRKLRVSAAGDLASDIRASNILTSAEGLRFELHTPWGSRPVQTHLIGR
FNVDNLLAVVAVLGALGESFERIVEAVSALHPVNGRMSRLGGHHGQPLVVVDYAHTPDAL
EQSLHAVRAHCEGRLICVFGCGGERDAGKRPLMGAIAERLADIAIITDDNPRGEDGDAIV
AQIVAGLMHPERAHVQRNRALAITEALHLAKPGDVVLIAGKGHETYQEGAHGKRPFDDLA
VARAALELHA