Protein Info for ABZR86_RS08740 in Dyella japonica UNC79MFTsu3.2

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 TIGR00435: cysteine--tRNA ligase" amino acids 3 to 456 (454 residues), 479.3 bits, see alignment E=7.7e-148 PF01406: tRNA-synt_1e" amino acids 17 to 314 (298 residues), 392.1 bits, see alignment E=3.7e-121 PF00133: tRNA-synt_1" amino acids 221 to 305 (85 residues), 33.3 bits, see alignment E=4.9e-12 PF09334: tRNA-synt_1g" amino acids 258 to 297 (40 residues), 23.4 bits, see alignment 4.8e-09 PF09190: DALR_2" amino acids 343 to 400 (58 residues), 48.9 bits, see alignment 1.6e-16

Best Hits

Swiss-Prot: 69% identical to SYC_XANCP: Cysteine--tRNA ligase (cysS) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 69% identity to xcc:XCC2251)

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2DID6 at UniProt or InterPro

Protein Sequence (460 amino acids)

>ABZR86_RS08740 cysteine--tRNA ligase (Dyella japonica UNC79MFTsu3.2)
MPITLYNSLTRRPEPFAPRDPQRVTMYVCGPTVYNYVHIGNARGPVVFDVLANLLRRHYP
RLVYARNITDVDDKINAAAKEAGCPISEITERYTEAYREDMAALGIAPPDLEPKATAHIE
PIIAMIESLIAGGHAYAAEGHVLFEVGSYPAYGELSGRDTEELIAGARVEVAPYKKNPGD
FVLWKPSTPDLPGWDSPWGVGRPGWHIECSAMCEAHLGDTIDIHAGGVDLQFPHHENEIA
QSTCAHGGKVFARWWLHNGMLTFDGRKMSKSLGNVLKVHELLKQHPAEALRLVLLRGHYR
QPLDWSDDAVAQAVSTLDGWYRVLRDLADVALPEVPLPVPAAVEAALCDDLNTPQALAEL
SQLADAARQADAAGRPAAKAALLGAGALLGLLQQDPEAWFKHGGATEVDAAQVESLLEQR
RAARAARDFKRADAIREELSAMGVVIEDGAQGTRWSVAKG