Protein Info for ABZR86_RS08705 in Dyella japonica UNC79MFTsu3.2

Updated annotation (from data): N-acetylglutamate synthase; N-acetylglutamate kinase (EC 2.3.1.1; EC 2.7.2.8)
Rationale: Important for fitness in most defined media, except when arginine is provided. Contains both acetylglutamate kinase (TIGR00761) and N-acetylglutamate synthase (PF04768) domains.
Original annotation: acetylglutamate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00761: acetylglutamate kinase
  • amino acids 22 to 242 (221 residues), 190.5 bits, E=1.7e-60
• PF00696: AA_kinase
  • amino acids 22 to 239 (218 residues), 98.8 bits, E=4.2e-32
• PF04768: NAT
  • amino acids 256 to 413 (158 residues), 178.4 bits, E=1e-56

Best Hits

Swiss-Prot: 79% identical to ARGB_XANCP: Acetylglutamate kinase (argB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00930, acetylglutamate/acetylaminoadipate kinase [EC: 2.7.2.- 2.7.2.8] (inferred from 79% identity to xal:XALc_1892)

Predicted SEED Role

"Acetylglutamate kinase (EC 2.7.2.8)" in subsystem Arginine Biosynthesis extended (EC 2.7.2.8)

MetaCyc Pathways

• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.1 or 2.7.2.- or 2.7.2.8

Sequence Analysis Tools

See A0A1I2DIM7 at UniProt or InterPro

Protein Sequence (421 amino acids)

>ABZR86_RS08705 N-acetylglutamate synthase; N-acetylglutamate kinase (EC 2.3.1.1; EC 2.7.2.8) (Dyella japonica UNC79MFTsu3.2)
MGSAKEIQQYLKRFSQLDAKRFAVVKVGGAVLRDDLPALTSSLTFLQQVGLTPIVLHGAG
PQLDEELSAAGIQKQTVNGLRVTSPKALAIVRKVFQEQNLRLVEALQGMDTRATSVPSGV
FTSEYLDRDVYGLVGKVSSINLAPIEASLRAGSIPVIASLGETAEGQILNINADFAANEL
VRVLQPYKIVFLTGTGGLLDDKGRIIDSINLSTEYEHLMAQPWINGGMRVKIEQIADLLS
SLPLTSSVSITQPSELAKELFTHKGSGTLVRRGEKVLRYESWEGIDLARMRELIESSFGR
KVVADYFERTRPYRIYVSENYRAAMILTQEEGLAYLDKFAVLDDAQGEGLGRAVWQVMRE
ENPQLFWRSRHGNQVNIFYYAESDGCFKQERWKVFWYGLKNFGEIERCVAHCAVRPATLM
D