Protein Info for ABZR86_RS08370 in Dyella japonica UNC79MFTsu3.2

Annotation: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 TIGR00174: tRNA dimethylallyltransferase" amino acids 10 to 296 (287 residues), 294.7 bits, see alignment E=3.4e-92 PF01715: IPPT" amino acids 43 to 286 (244 residues), 282 bits, see alignment E=4.7e-88

Best Hits

Swiss-Prot: 62% identical to MIAA_STRMK: tRNA dimethylallyltransferase (miaA) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 62% identity to sml:Smlt1735)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2DQ97 at UniProt or InterPro

Protein Sequence (339 amino acids)

>ABZR86_RS08370 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA (Dyella japonica UNC79MFTsu3.2)
MPVDSRHPGIFLMGPTASGKTALACALSKQFPVELVSVDSALVYRGLDIGAAKPDAATLA
RYPHALIDIRDPAEPYSAADFRADALQAMKDITARGKVPLLVGGTGLYFRALQQGLSQLP
EADPAIRERLAAQAGVIGWPALHERLREADPAAAARIGPNDAQRIQRALEVIELTGRPLS
EQQRGGSGERFPWRVLKLALLPVDRAPLHGRIAERFDAMLRDGFLDEMRGLRARGDLHPD
LPSMRAVGYRQAWEHLDGLTDAAAFRDRGIFATRQLAKRQITWLRGELDARTFDPDRPGL
EARVVDALELFLGTSRRGFSRPGSRFPGSLYETVSPARE