Protein Info for ABZR86_RS08015 in Dyella japonica UNC79MFTsu3.2

Annotation: tRNA pseudouridine(38-40) synthase TruA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 2 to 237 (236 residues), 207.2 bits, see alignment E=1.4e-65 PF01416: PseudoU_synth_1" amino acids 8 to 103 (96 residues), 41.2 bits, see alignment E=1e-14 amino acids 142 to 244 (103 residues), 98 bits, see alignment E=2.3e-32

Best Hits

Swiss-Prot: 66% identical to TRUA_STRMK: tRNA pseudouridine synthase A (truA) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 66% identity to sml:Smlt3424)

MetaCyc: 55% identical to tRNA pseudouridine38-40 synthase (Escherichia coli K-12 substr. MG1655)
tRNA-pseudouridine synthase I. [EC: 5.4.99.12]

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2DWK5 at UniProt or InterPro

Protein Sequence (255 amino acids)

>ABZR86_RS08015 tRNA pseudouridine(38-40) synthase TruA (Dyella japonica UNC79MFTsu3.2)
MRIALGIEYDGTDFLGWQRLTHGATVQGALEKALSFVAAHPVEVTCAGRTDAGVHGRCQV
VHFDTQVQRDPRGWVLGGCANLPRSVAVLWAQAVPEDFHARFSARSRRYRYRILNRPVRA
ALDGRYVTWERQPLDAARMHAAAQALVGEHDFSAFRAVSCQAAHARRAVLAVSVRREDEQ
VIVEIEANAFLHHMVRNIVGSLLPVGRGEQPVAWVGELLAGRDREVAGPTAPASGLTFLG
PRYEAHWGLPAEVSQ