Protein Info for ABZR86_RS07590 in Dyella japonica UNC79MFTsu3.2

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 4 to 860 (857 residues), 1239 bits, E=0
• PF00809: Pterin_bind
  • amino acids 26 to 264 (239 residues), 218.4 bits, E=2.4e-68
• PF02607: B12-binding_2
  • amino acids 323 to 396 (74 residues), 87.6 bits, E=1e-28
• PF02310: B12-binding
  • amino acids 416 to 506 (91 residues), 78.5 bits, E=7.7e-26
• PF02965: Met_synt_B12
  • amino acids 606 to 876 (271 residues), 425.1 bits, E=2.6e-131

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 64% identity to amc:MADE_02859)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• aspartate superpathway (21/25 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (8/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See A0A1I2FVD3 at UniProt or InterPro

Protein Sequence (895 amino acids)

>ABZR86_RS07590 methionine synthase (Dyella japonica UNC79MFTsu3.2)
MSTRHTRLSGLEPLVITPDLLFVNVGERTNVTGSAQFRKLIKEDRYEEAVDVARQQVASG
AQIIDVNMDEGLIDSEAAMTRFLNLIAAEPDIARVPVMVDSSKWTVLEAGLRCLQGKGIV
NSISMKEGEELFLEHARKVQQYGAAVVVMAFDEQGQADTCERKVEICSRAYALLTEQLDF
PPEDIVFDPNIFAIATGIEEHNNYAVDFIEATRELKRRFPLSHISGGVSNVSFSFRGNNT
VREAIHSVFLYHAIKAGMDMGIVNAGALMIYDDVPAELRERVEDVVLNRRPDATERLLEI
ADNYKARKGEVVVENLAWREKPVRERLSHALVHGIDAFVDADTEEARQLATRPLDVIEGP
LMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLLPYIEEEKARTGDVGKNNGTIVMA
TVKGDVHDIGKNIVGVVLRCNNFDVIDLGVMVPAQKILDAAREHNADLIGLSGLITPSLE
EMSHVAREMQRQEFSIPLLIGGATTSRAHTALKIDPHYKAPTVWVKDASRAVGVAQSLVS
KDLVDAFMAKVRADYEEVRERHRNRGPGKSLVPLEKARAQRYTCDWAGYAPPQPRQPGVT
VFDAYDLAELREYIDWTPFFQAWELAGRYPAILKDEIVGTQASELFRDAQAMLDRIVAER
WLTARAVIGFWRAAQVGDDTEVYGEDGRKLAVLRHLRQQADKPADRPDFSLGDFIAPKEA
GKQDWVGAFAVTAGIGIEEHVARFEAAHDDYSSILLKALADRLAEAFAERMHQRVRREFW
GYAPDEALDNEALIDEKYRGIRPAPGYPACPDHTEKSTLFKLLDATANAGIELTEGYAMY
PTAAVSGWYFSHPDSQYFVVGRLTREQVEDYAKRKGWTREEAERWLAPNLDYDPD