Protein Info for ABZR86_RS07520 in Dyella japonica UNC79MFTsu3.2

Annotation: thiamine pyrophosphate-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 PF00676: E1_dh" amino acids 64 to 371 (308 residues), 89.9 bits, see alignment E=2.4e-29 PF02779: Transket_pyr" amino acids 426 to 595 (170 residues), 64.5 bits, see alignment E=1.6e-21 PF02780: Transketolase_C" amino acids 632 to 738 (107 residues), 67.7 bits, see alignment E=1.4e-22

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 81% identity to psu:Psesu_1742)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2FWK8 at UniProt or InterPro

Protein Sequence (755 amino acids)

>ABZR86_RS07520 thiamine pyrophosphate-dependent enzyme (Dyella japonica UNC79MFTsu3.2)
MTALPFSLSARHKGFNRAEIVDQNFIEFVQLWQGHARAAPRDGEAVLPGSALDAQGFRDL
LESQLISRHLDLMARVLRVQNKVFYTIGSSGHEGNAMVARLTRHTDPAFLHYRSGGFMAE
RFRKLPGMDPVMDSALSFAASMEDPASGGRHKVWGSKPLWVLPQTSTIASHLPKAFGTAV
AIEQARRIGHPLPVPQDSIAICSFGDASSNHATAQTAFNAAAWTAYQKLPAPVLFVCEDN
GIGISVKTPSGWIANNFRQRPDLDYFHADGLDLAEGYGQVREAVEHCRRTRRPTFLHLRT
TRVMGHAGTDFEIEWRSVDELFALEATDPLLRSAEIALASGLYTRESLLALYEATRKQCF
AAAEEADRRPRLGTLAEVMKPLAPYTPAAVKAEAERADYAARRAEVFGGEDKLPEKQAPR
HLAIQIGQALHEVMAKYPESLLFGEDVALKGGVYTVTKGLFKTFKGNRVFNTLLDETVIL
GLAQGYANMGMLPMPEIQYLAYFHNACDQIRGEACSLQFFSNDQYRNPLVMRVAALGYQK
GFGGHFHNDNSIAALRDIPGLVVGCPSRGDDAAAMLRTLMALAKVDGRVCAFLEPIALYM
TKDLYEAGDGQWQFAYPAPGEALPLGEGRVYEQDADDLVVFTFGNGVPMALRAAREIEKK
HGWRTRVVDLRWLAPLNDKFIAGQARNARRILVLDEGRRSAGVGEGIITAIVEGGCGATP
LQRVVGADTYTPLAGAALLVLPGEAEIVAAAERML