Protein Info for ABZR86_RS07250 in Dyella japonica UNC79MFTsu3.2

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 4 to 792 (789 residues), 1192.3 bits, E=0
• PF01326: PPDK_N
  • amino acids 18 to 343 (326 residues), 423.5 bits, E=9.4e-131
• PF00391: PEP-utilizers
  • amino acids 382 to 454 (73 residues), 93.3 bits, E=8.8e-31
• PF02896: PEP-utilizers_C
  • amino acids 479 to 791 (313 residues), 207.4 bits, E=4.2e-65

Best Hits

Swiss-Prot: 69% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 82% identity to psu:Psesu_1439)

MetaCyc: 64% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• superpathway of anaerobic sucrose degradation (17/19 steps found)
• glycolysis II (from fructose 6-phosphate) (11/11 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)
• gluconeogenesis I (11/13 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See A0A1I2FX99 at UniProt or InterPro

Protein Sequence (795 amino acids)

>ABZR86_RS07250 phosphoenolpyruvate synthase (Dyella japonica UNC79MFTsu3.2)
MNDLVLWLDSLRMTDLGKVGGKNASLGEMIGNLAKLGVSVPGGFATTADAFQQYLDKSGV
AKRIQARLADLDVDDVDALTTAGKEIREWVTETPLPAELDQAIRAAYAKLCKDAGADDIA
VAVRSSATAEDLPDASFAGQQETFLNVVGIDDVLHKVKEVFASLYNDRAIAYRVHQGFKH
EDVFLSAGVQLMVRSDVGASGVLFTLDTESGFRDVVFVTGSYGLGEMVVQGAVNPDEFYV
FKPTLRQGKPAVLRRQLGAKQLRMVYSSAPGERVKTEPTPDELRHRFCISDQDVEELSKQ
ALIIEQHYGRPMDIEWAKDGNTGKLYIVQARPETVKSRAHATQLERFHLGEKGKVLAEGR
AIGQKIGAGKARVVRTLADMNKVQPGDVLVADMTDPDWEPVMKRASAIVTNRGGRTCHAA
IIARELGVPAVVGTGNALEKIPDGADVTVSCAEGDTGSIYEGILKFDRVTADLGAMPEAP
LKIMMNVANPERAFDFGQLPNAGIGLARLEMIIASHIGVHPKALLEYSKQDAATKAKIDE
RIAGYADPVSFYIDRLAEGIATIAASVFPKPVIVRLSDFKSNEYANLLGGSRYEPHEENP
MIGYRGASRYVDAGFAESFALECKAVKRVREVMGLDNVWVMIPFVRTLGEGRKVVEVLAK
NGLSQGASAHGEQALKIIMMCEVPSNALLADEFLEIFDGFSIGSNDLTQLTLGLDRDSSI
VASLFDERDPAVKKLLAMAIKTARDKGKYVGICGQGPSDHPDLAEWLMDQGIESVSLNPD
TVVDTWLRLAKKKAG