Protein Info for ABZR86_RS07165 in Dyella japonica UNC79MFTsu3.2

Annotation: translation elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 4 to 595 (592 residues), 968.9 bits, E=9e-296
• PF00009: GTP_EFTU
  • amino acids 4 to 181 (178 residues), 182.5 bits, E=1.3e-57
• TIGR00231: small GTP-binding protein domain
  • amino acids 7 to 175 (169 residues), 61.8 bits, E=6.6e-21
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 44.4 bits, E=3.6e-15
• PF00679: EFG_C
  • amino acids 399 to 477 (79 residues), 64.2 bits, E=1.8e-21
• PF06421: LepA_C
  • amino acids 487 to 593 (107 residues), 172.3 bits, E=5.3e-55

Best Hits

Swiss-Prot: 86% identical to LEPA_XANC5: Elongation factor 4 (lepA) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 86% identity to psu:Psesu_1990)

MetaCyc: 71% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A1I2FYC2 at UniProt or InterPro

Protein Sequence (596 amino acids)

>ABZR86_RS07165 translation elongation factor 4 (Dyella japonica UNC79MFTsu3.2)
MDLIRNFSIIAHIDHGKSTLADRIIQICGGLAEREMEAQVLDSNPIERERGITIKAQSVS
LPYKARDGKTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAV
EMGLEVVPVLNKIDLPTADPDKVKGEIEAVIGIDADDAVAVSAKTGQNVVEVLEAIVARI
PPPKPRDTDRLQALIIDSWFDNYLGVVSLVRVMQGEIRPGEKLLVMSTGRNHEVSEVGVF
TPKRKKLDKLGAGEVGWITASIKDVHGAPVGDTLTHAGKPADKPLPGFQTMQPRVFAGLF
PVAADDYPALREALEKLRLNDAALFFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYD
LDLVTTAPTVVYEILKSDGSVMLLDNPAKLPASPQVEEIREPIIVANILTPADYIGNIIT
LCEEKRGIQRSIQYLATQVQISYELPLAEVVLDFFDRLKSVSRGYASMDYHFERFDAGPF
VRTDVLINGDRVDALSLIVHRSHADRRGRDLVERMKELIPRQQFDVAIQAAIGAQVIARS
TVKALRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLKVDK