Protein Info for ABZR86_RS06005 in Dyella japonica UNC79MFTsu3.2

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 9 to 693 (685 residues), 1078 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 143 (130 residues), 93.6 bits, see alignment E=3.4e-30 amino acids 325 to 457 (133 residues), 93.7 bits, see alignment E=3.1e-30 PF03725: RNase_PH_C" amino acids 146 to 209 (64 residues), 50.7 bits, see alignment E=3.5e-17 amino acids 461 to 530 (70 residues), 29.8 bits, see alignment E=1.2e-10 PF03726: PNPase" amino acids 241 to 321 (81 residues), 59.1 bits, see alignment E=1.3e-19 PF00013: KH_1" amino acids 558 to 615 (58 residues), 45.7 bits, see alignment 1.1e-15 PF00575: S1" amino acids 620 to 691 (72 residues), 73.7 bits, see alignment E=3.2e-24

Best Hits

Swiss-Prot: 78% identical to PNP_XANCP: Polyribonucleotide nucleotidyltransferase (pnp) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 80% identity to psu:Psesu_1825)

MetaCyc: 66% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I1XWG4 at UniProt or InterPro

Protein Sequence (699 amino acids)

>ABZR86_RS06005 polyribonucleotide nucleotidyltransferase (Dyella japonica UNC79MFTsu3.2)
MAKVTKSFQYGNHTVTLETGEIARQASGAVMVSMDGTVVLVTAVAAAKAKDGQDFFPLTV
DYVEKFYSAGRIPGGFFKREGRPTEKETLTSRLIDRPVRPLFPEEFKNEVQVIAQVVSLN
PEVDGDIPAMLGASAALSLAGIPFKGPIGAARVGYVGGKYVLNPTATELKGSDLDLVVAG
TTNAVLMVESEAKLLSEEVMLGAVVYGHQQMQVAIRAIAELATEAARPSMGWQPPVRNEA
LAAAVQGAVGNKLEEAFQVRDKLQRRDAIAAIKADVLASLKAQAEEHAWPSAELAKEFGE
LEYRTMRDSVLKTKVRIDGRQLDDVRPITVRVGVLPRTHGSALFTRGETQALVVATLGTT
RDAQIIDAPEGESKDPFLFHYNFPPFSVGEAGRFGAPKRREIGHGRLAKRGVQAVKPSIE
EFPYVLRVVSEITESNGSSSMASVCGSSLAMMDAGVPLKAPVAGIAMGLVKEGGDYVVLS
DILGDEDHLGDMDFKVAGSATGVSALQMDIKIDGITEEIMKVALEQAKRGRLHILGEMAK
VISEPRSEMSEYAPRLLTIKIHPDKIREVIGKGGATIRSITEETGTTIDISDDGTVIIAS
VNRLAADDAKKRIEQIVSDVEPGRIYEGKVAKLMDFGAFVTILPGKDGLVHVSQISNERV
EKVSDKLKEGDIVKVKVLEVDKQGRIRLSMKAVTEEEGA