Protein Info for ABZR86_RS05975 in Dyella japonica UNC79MFTsu3.2

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 838 (831 residues), 1225 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 505 (474 residues), 560 bits, see alignment E=3.6e-172 PF03989: DNA_gyraseA_C" amino acids 537 to 584 (48 residues), 44.6 bits, see alignment 7.9e-16 amino acids 587 to 636 (50 residues), 32.5 bits, see alignment 4.7e-12 amino acids 644 to 687 (44 residues), 38.5 bits, see alignment 6.1e-14 amino acids 692 to 737 (46 residues), 48.4 bits, see alignment 5e-17 amino acids 743 to 788 (46 residues), 40.9 bits, see alignment 1.1e-14 amino acids 794 to 838 (45 residues), 44.9 bits, see alignment 6e-16

Best Hits

Swiss-Prot: 63% identical to GYRA_SALTY: DNA gyrase subunit A (gyrA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 65% identity to hch:HCH_04983)

MetaCyc: 64% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I1XVW7 at UniProt or InterPro

Protein Sequence (889 amino acids)

>ABZR86_RS05975 DNA gyrase subunit A (Dyella japonica UNC79MFTsu3.2)
MAELAKEIIRVNIEDEMRQSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNELSNVWN
KPYKKSARVVGDVIGKYHPHGDASVYDAIVRMAQPFSLRYMLVDGQGNFGSVDGDNAAAM
RYTEVRMARLTHELLADIDKETVDFGPNYDESESEPLVLPTRVPNLLVNGSAGIAVGMAT
NVPPHNLNEVIAATIGLIDEPSLSVDDLMQYIPGPDFPTAGIINGSSGIVEAYRTGRGRI
LVRARAEIETESNGRETIIVTELPYQVNKARLIEKIAELVKEKKLEGISELRDESDKDGM
RVVIEIRRDSMADVVLNNLFQQTQLQVTFGINMVALLDGQPRLLNLKDILEAFIRHRREV
VTRRTIFELRKARARAHILEGLTVALANIDEMIELIRNSASPQEARERMLARKWQPGMVS
ALLAAAGADASRPEDLDPRDGLKADGYQLSDVQAQEILAMRLHRLTGLEQEKLSDEYREI
LETIRGLIEILEDPARLLAVIRAELEVIKEEFGDKRRTEIQHSQEDLNVLDLIAPEDVVV
TLSHTGYVKRQPASTYRAQRRGGKGRSASALKDEDFVEQLWVVNTHDTLLTFTSTGRVYW
LNVYQMPESGPNARGKPMVNLLPLAAGEKVQAVLPVREYSDDRYVFFATRQGTVKKTPLN
EFAFQLQKGKIAINLDEGDALVNVELTDGNSDVLLFASNGKTVRFDEGEVRSMGRTATGV
RGMKLADGAEVVSLIVVAPEGGDILTATARGYGKRTLLEEFPKKGRGTQGVIGIQCSERN
GGLVSAVQVTEAHELMLISNQGTLVRTRVAEVSQLSRNTQGVTLIKLPADEALVSVVRLD
AEEESEGSGAEALDTDGGSVEAGEVVPGSNDAVEDGAGESGTDDSEPEA