Protein Info for ABZR86_RS05890 in Dyella japonica UNC79MFTsu3.2

Annotation: bifunctional sulfate adenylyltransferase/adenylylsulfate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14306: PUA_2
  • amino acids 19 to 177 (159 residues), 183.8 bits, E=3.7e-58
• TIGR00339: sulfate adenylyltransferase
  • amino acids 21 to 400 (380 residues), 461.3 bits, E=2.2e-142
• PF01747: ATP-sulfurylase
  • amino acids 186 to 400 (215 residues), 281.6 bits, E=6.9e-88
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 400 to 561 (162 residues), 181.7 bits, E=1.1e-57
• PF01583: APS_kinase
  • amino acids 408 to 561 (154 residues), 201.9 bits, E=1.2e-63
• PF13671: AAA_33
  • amino acids 413 to 528 (116 residues), 30.5 bits, E=8.4e-11

Best Hits

Swiss-Prot: 56% identical to MET3_GIBZE: Sulfate adenylyltransferase (MET3) from Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084)

KEGG orthology group: K00958, sulfate adenylyltransferase [EC: 2.7.7.4] (inferred from 64% identity to rmr:Rmar_1856)

Predicted SEED Role

"Sulfate adenylyltransferase, dissimilatory-type (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.25, 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See A0A1I1XUM0 at UniProt or InterPro

Protein Sequence (585 amino acids)

>ABZR86_RS05890 bifunctional sulfate adenylyltransferase/adenylylsulfate kinase (Dyella japonica UNC79MFTsu3.2)
MSQSALASLPIEPELEPLIAPHGGTLKDLYLPYPEAQALKQRSAGLPALDLNTRQLCDLE
LLLNGAFSPLEGFLTEADYRRVVDELRLADGTLWPIPITLDVSEDFAATLASGAELVLRD
AQGVPLAVLEVQDIYRPDREHEARQVFGTLDHAHPGVAELLERTRPVYLGGRLRGIQAPV
HYDFGELRDTPRSLRAWFEANGWNRIVAFQTRNPMHRAHRELTLRAAQKVGAKLLIQPSV
GRTKPGDVDHYTRVRCYRALLPQYPADSARLSLLPLAMRMGGPREAIWHAIIRKNYGATH
FIVGRDHAGPGKDSSGQPFYGPFDAQHLLERYQEELGIQVVAFPAMVYAANRDAYVPATE
VAADDEVRDISGTQLRRHLHEGTDIPAWFTFPEVVDILRERHPARAPRGFALFFTGLSGA
GKSTIAQAVVAQLLEHTSRAVTVLDGDEVRKHLSRGLGFSREDRAANVTRIGYVASEIVR
HGGVAVCAPIAPYADARAEARGLVEAHGDFIEIHVSTPLEVCEERDRKGLYAQARAGKIA
QFTGISDPYEVPEQPEIRVDGSGAAPAVLAGHVLGLLKDRGLLAG