Protein Info for ABZR86_RS03865 in Dyella japonica UNC79MFTsu3.2

Updated annotation (from data): Nitrate reductase (EC 1.7.99.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.99.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 903 PF04879: Molybdop_Fe4S4" amino acids 4 to 54 (51 residues), 57.2 bits, see alignment 2.7e-19 PF00384: Molybdopterin" amino acids 59 to 489 (431 residues), 233.1 bits, see alignment E=9.9e-73 PF01568: Molydop_binding" amino acids 597 to 708 (112 residues), 96.8 bits, see alignment E=1.6e-31 PF04324: Fer2_BFD" amino acids 846 to 896 (51 residues), 50.5 bits, see alignment 4.1e-17

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 66% identity to rso:RSp1219)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I1Z438 at UniProt or InterPro

Protein Sequence (903 amino acids)

>ABZR86_RS03865 Nitrate reductase (EC 1.7.99.4) (Dyella japonica UNC79MFTsu3.2)
MSEAVRTTCPYCGVGCGVLASVDADGTTRVAGDPAHASNLGRLCVKGSALGDTVGPEGRL
LHPVLRGQDGVAQRATWDEAIQRVADGFSRIVAEHGPDAVAFYVSGQLLTEDYYVVNKLA
KGYLGTANIDTNSRLCMSSAVAGHKRAFGEDVVPGCYEDLEQAELVVLVGSNTAWCHPVL
FQRVTAAREGRDGPRVVVIDPRFTATCEGADLHLPIRPGTDVRLFNGLLSFLFRHGVIDP
DFIDAHTAGFDQALAAAEAESGDPAMVARQCGLQTADVVAFYRLFARHEKVVTLFSQGVN
QSTSGTDKVNAIINCHLATARIGRPGMGPFSITGQPNAMGGREVGGLSNMLAAHLELGEP
AHRDIVQAFWQSPHIAGTPGLKAVELFDAVGEGRIKALWIMATNPAVSLPDADKVRAALA
RCELVVCSDIVERTDTNAYAHVLLPALGWGEKDGTVTSSERRISRQRPFLPAPGEARADW
RAVCEVAKRMGFERGFEFAHPHEIFVEHAQLTAFRNREPLADGVPAARRQLQLGPLAKLD
RRAYDALAPVQWPVTEQGAGTPRLFADGRFSHADGRARFVTTASRAPAHAADREYPLVLN
TGRIRDQWHTMTRTARSPRLNNHIGEPFVDMHPHDALRSGVREGSLARVVSRWGSMVARV
RHGGGILAGTVFAPIHWSDQFASDARVGGVVNPAVDPVSGEPEFKHTPVRVEPFPVRWYG
FALARRPLPASMLSYWTLIRGDRFLRYELAHRERPAEIEAWAKAWLGVTDDQADWLAFED
LDTGVYRAVHLREGRIESCLFLAAQPHLPSRQWLASLFARDRLDEADRLALLSGEAPGGG
GDPGPVVCSCFGVGRNTILEVIRRNELDLPAQITAELRAGGNCGSCIPELRRLIAETRAA
SEA