Protein Info for ABZR86_RS01875 in Dyella japonica UNC79MFTsu3.2

Annotation: NAD-dependent DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 PF01653: DNA_ligase_aden" amino acids 10 to 329 (320 residues), 400.2 bits, see alignment E=2.2e-123 TIGR00575: DNA ligase, NAD-dependent" amino acids 14 to 614 (601 residues), 686.8 bits, see alignment E=1.5e-210 PF03120: DNA_ligase_OB" amino acids 332 to 408 (77 residues), 112 bits, see alignment E=3.5e-36 PF03119: DNA_ligase_ZBD" amino acids 418 to 445 (28 residues), 48 bits, see alignment (E = 3.1e-16) PF12826: HHH_2" amino acids 538 to 601 (64 residues), 54.2 bits, see alignment E=4.5e-18 PF00533: BRCT" amino acids 715 to 786 (72 residues), 56.4 bits, see alignment E=1e-18 PF12738: PTCB-BRCT" amino acids 730 to 777 (48 residues), 26.8 bits, see alignment 1.5e-09

Best Hits

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 64% identity to psu:Psesu_1734)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2ACU0 at UniProt or InterPro

Protein Sequence (798 amino acids)

>ABZR86_RS01875 NAD-dependent DNA ligase LigA (Dyella japonica UNC79MFTsu3.2)
MSHSSDPKVHAAELREQIERANYQYHVLDDPRITDIEFDRLLRELEALETAHPELATPDS
PTRRVGARAVGGFAEVQHAIPMLSLSNAFEQEGDTELERFREVAEFERRIEQTLQRREPV
FSVEPKLDGLAISLRYEDGVFVQGATRGDGATGEDVTANLRTVDAIPLRLRGKDWPRVLE
VRGEVIMLRKDFEAFNAHAREHGEKVLANPRNGAAGSLRQLDPAITKRRKLSFFAYAIGV
VEGGTIPDTHSATLQKLRDWGFPVSHEVGTAKGFKGLLAYYRRIGEKRDSLPFDIDGVVY
KLDDYEGQREMGFVSRAPRWAIAHKFPAQEQTTTVEAIEINIGRTGAATPLARLKPVQVA
GVTVSNATLHNADQVARLDVRVGDTVIVRRAGDVIPEVVRVMSELRPPHTKAWHMPSHCP
VCNSELLREEGEAVWRCSGGLICPAQRKEALIHFAARRAMDIEGIGERFVDALVDFGYVH
TPADLYQLTLDDFLEMKRRADERDGTTPETVKQGKVATKWAENLLEAIEASKRSTLPRFL
YALGIMHIGESTAKTLASWLGRLELVRSTPAPVLRVLPDIGDEVANSIDGFFAQEGNQRV
VDALLAAGIELGDESAPSPKLRGKLGLGVLLDTAKVSKLGPKSTQQLAQHYRSLKQLADA
GASHWVVAGVPSAAATNLELFLADQENRKALEEAEAAMQRLLDAIPKSAKAEAAPLEGQT
VVLTGTLAGLTRDEAKERLEALGAKVSGSVSKKTSFVVAGTEAGSKLDKAQELGVEVWDE
ARLLALLAEHESEGDLWI