Protein Info for ABZR86_RS00975 in Dyella japonica UNC79MFTsu3.2

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 TIGR00392: isoleucine--tRNA ligase" amino acids 16 to 856 (841 residues), 881.7 bits, see alignment E=3e-269 PF00133: tRNA-synt_1" amino acids 28 to 656 (629 residues), 471.9 bits, see alignment E=5.1e-145 PF09334: tRNA-synt_1g" amino acids 60 to 202 (143 residues), 37 bits, see alignment E=5.4e-13 amino acids 592 to 648 (57 residues), 22.4 bits, see alignment 1.5e-08 PF13603: tRNA-synt_1_2" amino acids 309 to 369 (61 residues), 25.6 bits, see alignment 2.4e-09 PF01406: tRNA-synt_1e" amino acids 592 to 663 (72 residues), 32.2 bits, see alignment 2.2e-11 PF08264: Anticodon_1" amino acids 700 to 855 (156 residues), 91.9 bits, see alignment E=1.1e-29 PF06827: zf-FPG_IleRS" amino acids 915 to 942 (28 residues), 31.9 bits, see alignment (E = 2.7e-11)

Best Hits

Swiss-Prot: 54% identical to SYI_PSEA6: Isoleucine--tRNA ligase (ileS) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 55% identity to gag:Glaag_1154)

MetaCyc: 59% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2CNJ7 at UniProt or InterPro

Protein Sequence (953 amino acids)

>ABZR86_RS00975 isoleucine--tRNA ligase (Dyella japonica UNC79MFTsu3.2)
MTQDYKNTINLPQTDFPMRGDLPKREPQWLAEWEKLGRYAQIQQRTAGRDKVFVLHDGPP
YANGAIHLGHAVNKVLKDVVVKSRLLAGYRSPYVPGWDCHGLPIEIAVEKKHGKAGDKLD
AVAFRQKCREYAQQQIDLQRTDFKRLGVLGDWENPYRTMAFTFEADMLRALARIVDNGHV
VRGFKPVYWCFDCGSALAEAEIEYADKVSPAIDVAYDALDPHALAAKFGVEAGDAIVAIP
IWTTTPWTLPESQAVSMGAELEYALVEGPARDGRRVLLVLASALVEKASHRYGVEHPVVL
GHVAGQALEGVLLQHPFYDRRIPVLLGEHVSAEDGTGAVHTSPDHGVEDFVVSRKYGIET
LNYVEPRGTYRADTPPAGELTLAGTHLWKANDAIVDMLRGRGVLLAHHKIEHSYPHCWRH
KTPVIFRATPQWFISMEQAGLRKAAMESIRQVRWVPGWGEERIAGMVGDRPDWCISRQRT
WGVPIALFVHKGTQEPHPRSVELLEQVAKRVEQGSIDAWYALDPAELLGDEAKDYEKVND
VLDVWFDSGVTHFAVVGQRPELQQGDAATYKVMYLEGSDQHRGWFQSSLLTSAAIHGRAP
YDDVLTHGFTVDAQGRKMSKSLGNGIEPQDIMKNLGADILRLWICSTDYRNEMSLSDEIL
KRVSDMYRRIRNTARFLLGNLDGFDPAQHLLPVEDCLLLDQWAVQQAYDTQQAVAAAYDR
YDFPEIVQRVQNFCTTELGALYLDITKDRLYTMPTDSRGRRSAQSAMYRIAEALVRWLAP
VLSFTAEEIWKALPGQRGESVLFETWYDGLAATQGSPEQRRYWSDLLAIRDTASRVLEGM
RKAEQIGASLEAKLAIHADPALVSRYAPAADELRFFFITSDLRLDVAGGQPDTAVLSELE
GADVWVSATVSDAAKCVRCWHRRDDVGQHAAHPELCGRCVSNVDGPGEDRRWF