Protein Info for ABIE53_006157 in Paraburkholderia graminis OAS925

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 3 to 999 (997 residues), 1426.1 bits, see alignment E=0 PF13510: Fer2_4" amino acids 18 to 101 (84 residues), 82.6 bits, see alignment E=1.1e-26 PF07992: Pyr_redox_2" amino acids 170 to 430 (261 residues), 44.6 bits, see alignment E=8.4e-15 PF01134: GIDA" amino acids 170 to 222 (53 residues), 19.4 bits, see alignment 3.1e-07 PF12831: FAD_oxidored" amino acids 170 to 232 (63 residues), 42.3 bits, see alignment 4.4e-14 PF00890: FAD_binding_2" amino acids 170 to 210 (41 residues), 26 bits, see alignment (E = 3.6e-09) PF13450: NAD_binding_8" amino acids 173 to 215 (43 residues), 32.5 bits, see alignment (E = 5.6e-11) PF17806: SO_alpha_A3" amino acids 518 to 600 (83 residues), 109 bits, see alignment E=7.5e-35 PF01571: GCV_T" amino acids 615 to 879 (265 residues), 244.2 bits, see alignment E=8.5e-76 PF08669: GCV_T_C" amino acids 905 to 991 (87 residues), 71 bits, see alignment E=4.1e-23

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 98% identity to bug:BC1001_5827)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1000 amino acids)

>ABIE53_006157 sarcosine oxidase subunit alpha (Paraburkholderia graminis OAS925)
MSQKDRLPNGGRINRALPLTFTFNGRQYQGYQGDTLASALLANGLHFVARSWKYHRPRGI
VTAGVEEPNAVVQLETGAYTVPNARATEVELYQGLVANSVNAKPSIEKDRMAVNQKIARF
IPAGFYYKTFMWPRKFWPKYEEVIRDAAGLGKAPEQLDADRYDKCFAHCDVLVVGGGPTG
LAAAHAAALSGARVTLVDDQPELGGSLLSCRAEIDGKPALQWVHKIEDELRQMPGVKILS
RSTAFGYQDHNLVTVTQRLTDHLPVSQRKGTRELMWKIRAKRVILATGAHERPIVFGNND
LPGVMLASAVSTYLHRYAVLPGRDAIVFTNNDDGYQCALDLKAAGAQVTVIDPRAGESKG
TLPALARRYGVKVISGAVITAAHGKLRVASVDVASYANGQVGAKQGELPCDLLAMSGGWS
PVLHLFAQSGGKAHWHDDKACFVPGKVMQPETSVGACNGDFTLGQGIRFAVDAGVEAARA
AGYIVKRPNTVQVAEITEAKMQPLWLVGGRELATRGPKQFVDFQNDVSAADIFLAAREGF
ESVEHVKRYTAMGFGTDQGKLGNINGMAILAQALGKTIPETGTTTFRPNYTPVTFGTFAG
RELGEFLDPVRKTAVHEWHVENGAEFEDVGNWKRPWYFPKKGEDLHAAVARESLAVRTSV
GILDASTLGKIDIQGPDSAKLLNWVYTNPWSKLEVGKCRYGLMLDENGMIFDDGVTVRLA
DRHYMMTTTTGGAARVLTWLERWLQTEWPDMRVRLASVTDHWATFAVVGPNSRKVLQKVC
HDIDFANAAFPFMSYREGTVAGAASRVMRISFSGELAYEVNVPANVGRAVWEALMAAGAE
FDITPYGTETMHVLRAEKGYIIVGQDTDGSMTPHDVGMGGLVAKSKDFLGKRSLTRSDTA
KAGRKQLVGLLADDPSFVIPEGSQIVAGPFQGETAPMLGHVTSSYYSPILKRSIAMAVVK
GGLDKIGETVTVPLSSGKQIAAKITSSVFYDSEGARQHVE