Protein Info for ABIE53_005813 in Paraburkholderia graminis OAS925

Annotation: DHA2 family multidrug resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 530 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 70 to 90 (21 residues), see Phobius details amino acids 99 to 122 (24 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details amino acids 185 to 207 (23 residues), see Phobius details amino acids 219 to 238 (20 residues), see Phobius details amino acids 248 to 268 (21 residues), see Phobius details amino acids 288 to 310 (23 residues), see Phobius details amino acids 324 to 342 (19 residues), see Phobius details amino acids 353 to 369 (17 residues), see Phobius details amino acids 381 to 404 (24 residues), see Phobius details amino acids 420 to 434 (15 residues), see Phobius details amino acids 495 to 515 (21 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 32 to 514 (483 residues), 489.7 bits, see alignment E=4.9e-151 PF07690: MFS_1" amino acids 37 to 431 (395 residues), 179 bits, see alignment E=1.3e-56 PF06609: TRI12" amino acids 38 to 311 (274 residues), 28.2 bits, see alignment E=7.2e-11

Best Hits

Swiss-Prot: 52% identical to FARB_NEIGO: Fatty acid resistance protein FarB (farB) from Neisseria gonorrhoeae

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 96% identity to bug:BC1001_3774)

MetaCyc: 47% identical to tripartite efflux pump membrane subunit EmrY (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (530 amino acids)

>ABIE53_005813 DHA2 family multidrug resistance protein (Paraburkholderia graminis OAS925)
MSQSTSAESPAPAPTSAPPAPPPPLKGGQLVLATIAVALATFMNVLDTSIANVAIPTISG
NLGVSVDEGTWVITVFAAANAVSIPLTGWLTQRIGQVKLFVGAILMFVIASWLCGIAPTL
PILLLARVFQGAVAGPLIPLSQAILLGSYPKEKASTALSLWAMTATVGPIAGPALGGWIT
DSYSWSWIFYINIPVGLFAAGVTWMIYRTRESPTRKPPIDVVGLGLLITWVASLQIMLDK
GKDLDWFASPVIVILGLTALVSFAFFLVWELTEENPIIDLRLFRERNFLGGTIAISVAYG
VFFGNLVLLPQWMQEYLNYRSVDAGLVTAPLGIFAVILAPVMGRVLPRSDARIISTMAFV
GFAIVFYMRSKYVIEIDTWHLVIPTLLQGIPMALFFVPLTSIILSGQPPNKIPAAAGLSN
FVRVFCGAVGTSVAGNAWNNRTVLHHERLTERASVDNPLFNQQIDSTQSLLHLNTQSAHA
LFDFTVNTQAAMMGLNDIFFVSAVIFILIIPLIWITRPAKGGGGDAAGAH