Protein Info for ABIE53_005639 in Paraburkholderia graminis OAS925

Annotation: isoquinoline 1-oxidoreductase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 753 transmembrane" amino acids 28 to 48 (21 residues), see Phobius details PF20256: MoCoBD_2" amino acids 64 to 208 (145 residues), 41.7 bits, see alignment E=1.1e-14 amino acids 613 to 692 (80 residues), 56.8 bits, see alignment E=2.6e-19 PF02738: MoCoBD_1" amino acids 341 to 573 (233 residues), 114.3 bits, see alignment E=5.2e-37

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 73% identity to bph:Bphy_6800)

MetaCyc: 72% identical to 2-deoxy-D-ribose dehydrogenase beta subunit (Pseudomonas simiae)
1.1.2.M2 [EC: 1.1.2.M2]

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.1.2.M2 or 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (753 amino acids)

>ABIE53_005639 isoquinoline 1-oxidoreductase beta subunit (Paraburkholderia graminis OAS925)
MSKMVFEPVSDETFPTTETPVNISRRRFLLASAGIAGGALVLGFGLPVREARAQAATNLP
IKRVAAFLEIRPDNTIRFQSPFIEGGQGIFTAMAQIIGEELDADPATFVVENAPPGKDYQ
IMDSGGRYTGGSSSVRSSYQTMRRLGALTRGMLLQAAARQLHVPVSQLTTQPGRVVHSGS
NRSLSYGQLASSAINLPVPDPDSVKLKDPTQFRWIGKPIERLDVHEKSTGRAIYAIDTHV
DGMLHASVQHAPRLGLTVGSLRNESQVKAMKGVHSVHRLDGAVAVVAERWWVAKRAAEAL
QVDWREPGPDSTLRYMPADFSTNAFREQLASASGSGDTAETAGDVGSAMKHAHATVSATY
HSQYLNHAQLEPPSSLARFNPDGTLEVWLPNQAPDLFLADMAKRTGLDPQKIIIHSPILG
GFFGRHFIYNTASPYPQAVQLAKETGRPVKVIWSREEEFLRDILRPMAVVQFRAGLDANG
MPVALEAVSATEGPTEGINNKRDTKLDPTALEGLTGKAYAIPHRRIAQLYVKTPVMLGYW
RSVGNSHNDFFYETFLDEMADKGGQDPYDLRLRLLQGNRRLTNLLRAVSELAGGWQRGPF
TAKDGTRRARGIAMASPFGSQTAAIAEVSIMDGQVVVHDIWQAIDPGNIVNPAIVEAQVS
GGVALGLSQILVEEAVYENGMPVARNYDMYPILPPRRMARVHVRVVESGEKMGGIGEPPV
PAVAPAVANAVSRLTGQRVRSMPLSRYSFKNQA