Protein Info for ABIE53_005122 in Paraburkholderia graminis OAS925

Annotation: 6-phosphogluconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03446: NAD_binding_2
  • amino acids 6 to 172 (167 residues), 170.2 bits, E=3.8e-54
• TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)
  • amino acids 6 to 469 (464 residues), 737.1 bits, E=4.3e-226
• PF00393: 6PGD
  • amino acids 179 to 468 (290 residues), 447.8 bits, E=1.8e-138

Best Hits

Swiss-Prot: 74% identical to 6PGD_SALTY: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 98% identity to bug:BC1001_5224)

MetaCyc: 73% identical to 6-phosphogluconate dehydrogenase, decarboxylating (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.44

Use Curated BLAST to search for 1.1.1.44

Protein Sequence (469 amino acids)

>ABIE53_005122 6-phosphogluconate dehydrogenase (Paraburkholderia graminis OAS925)
MGKQAIGVVGLAVMGRNLALNIESRGHAVSVYNRSREKTDELIAEYPDKKLVPAFTLEEF
VESLEKPRRILLMVKAGEPTDATIASLKPLLEKGDILIDGGNTHFTDTIRRNQELAKAGL
HFIGTGVSGGEEGALKGPSIMPGGQRDAYDLVAPILTEIAAKAPDGEPCVAYMGPDGAGH
FVKMVHNGIEYGDMQLIAESYAVLKQVAGLSNEELGKVYTEWNQGELDSYLIEITSKIFS
KKDEETGKDLVDVILDRAAQKGTGKWTSQNALDLGAPLPLITEAVFARVLSSLKSQRVAA
SKVLDGPKAKPLGAEREAFIESVRRALYFSKVISYAQGFAQLRAASEEYKWDLDFGTIAK
IFRAGCIIRARFLQKITDAYTKDKAIANLLLDPYFRDIASNYQTALREVVVAAINAGVPV
PAFASAIAYFDAYRSERLPANLVQAQRDFFGAHTFERVDKPGSFHANWS