Protein Info for ABIE53_005023 in Paraburkholderia graminis OAS925

Annotation: tartrate dehydrogenase/decarboxylase/D-malate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02089: tartrate dehydrogenase
  • amino acids 3 to 358 (356 residues), 549.7 bits, E=1.3e-169
• PF00180: Iso_dh
  • amino acids 7 to 355 (349 residues), 310.6 bits, E=7.8e-97

Best Hits

Swiss-Prot: 70% identical to DMLA_ECOLI: D-malate dehydrogenase [decarboxylating] (dmlA) from Escherichia coli (strain K12)

KEGG orthology group: K07246, tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase [EC: 1.1.1.83 1.1.1.93 4.1.1.73] (inferred from 99% identity to bug:BC1001_5137)

MetaCyc: 70% identical to D-malate/3-isopropylmalate dehydrogenase (decarboxylating) (Escherichia coli K-12 substr. MG1655)
Tartrate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73]; 3-isopropylmalate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85]; D-malate dehydrogenase (decarboxylating). [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85, 1.1.1.83]

Predicted SEED Role

"Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83)" (EC 1.1.1.83, EC 1.1.1.93, EC 4.1.1.73)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• methylgallate degradation (5/6 steps found)
• D-malate degradation (1/1 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (6/8 steps found)
• gentisate degradation II (2/4 steps found)
• superpathway of vanillin and vanillate degradation (6/10 steps found)
• syringate degradation (7/12 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• Glyoxylate and dicarboxylate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.83, 1.1.1.85, 1.1.1.93, 4.1.1.73

Use Curated BLAST to search for 1.1.1.83 or 1.1.1.85 or 1.1.1.93 or 4.1.1.73

Protein Sequence (360 amino acids)

>ABIE53_005023 tartrate dehydrogenase/decarboxylase/D-malate dehydrogenase (Paraburkholderia graminis OAS925)
MSKKYRIAVIPGDGIGVEVMPEALRVLDAVKTRFGIELEYQHIEWASCDYYAKHGQMMPD
DWKAQLQSADAILFGAVGWPDTVPDHVSLWGSLLKFRREFDQYINLRPARLFAGVPSPLA
GRKAGDIDFWIVRENTEGEYSSVGGVMFEGTEREFVLQESVFTRHGSERVLKFAFDLAQR
RERKKITVATKSNGIAISMPWWDKCAAGIAAQYPDVTWDKQHIDILCARFVLNPDRFDVV
VATNLFGDILSDLGPACTGTIGLAPSGNLNPDRKFPSLFEPVHGSAPDIAGKNIANPIAM
IWSAAMMLDFLGNHEGKEREAHDAILAAIEATLVEGPHTGDLGGKANTTQVGEAIAARLG