Protein Info for ABIE53_004865 in Paraburkholderia graminis OAS925

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 624 TIGR01391: DNA primase" amino acids 1 to 428 (428 residues), 451.6 bits, see alignment E=1.2e-139 PF01807: zf-CHC2" amino acids 4 to 97 (94 residues), 130.1 bits, see alignment E=7e-42 PF08275: DNAG_N" amino acids 134 to 261 (128 residues), 131.1 bits, see alignment E=8.1e-42 PF13662: Toprim_4" amino acids 270 to 338 (69 residues), 61 bits, see alignment E=3.3e-20 PF01751: Toprim" amino acids 271 to 341 (71 residues), 50.4 bits, see alignment E=6.3e-17 PF13155: Toprim_2" amino acids 273 to 359 (87 residues), 64.3 bits, see alignment E=3.4e-21 PF10410: DnaB_bind" amino acids 379 to 433 (55 residues), 37.2 bits, see alignment 8.5e-13

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 98% identity to bug:BC1001_4960)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (624 amino acids)

>ABIE53_004865 DNA primase (Paraburkholderia graminis OAS925)
MIPPSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CGAHGTAIGFLMEHVGASFPEAVNELAQSVGLTVPNEPSPGYGGAGGSGGGYSPAASKAV
TTALSDVMQTACDYYRKQLRGAPVAIQYLKKRGLTGEIAARFGLGYAPDGWQNLEAAFPN
YRDDSLVESGLVIVSEKSDAQGQNRRYDRFRERIMFPIRNVKGQVIGFGGRVLDGGEPKY
LNSPETPLFNKGSELYGLFEARLAIREQGYVLVVEGYMDVVALAQLGFQNAVATLGTACT
PVHVQKLMRQTDTVIFSFDGDSAGRRAARRALDACLPHAADNRTIRFLFLPTEHDPDSYV
REFGTEAFAEQVERAMPLSQFMLNEVLAGKELDQPEGRARALFDAKPLLQALPANALRAQ
IMHMFADRLDVPFDEVAALCEVDSRIAAAARLAPARKDRRSVTGIEEKTLRNLVMYPRTV
AVLDEEAEQALLTVTRHGELFEEVTTHARALGDSAEFQLLSDLLRNGANAPTFEEIFRKI
LDYDENVRDLLLKDPEDATVIEERREQERIVGEELKAAILKMRYDAYCDRLEQLSRQSRH
TPEEFAELAELNRKRADMKRQLGL