Protein Info for ABIE53_004717 in Paraburkholderia graminis OAS925

Annotation: peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 533 TIGR00503: peptide chain release factor 3" amino acids 3 to 524 (522 residues), 682 bits, see alignment E=4.7e-209 PF00009: GTP_EFTU" amino acids 7 to 272 (266 residues), 174.1 bits, see alignment E=6.1e-55 PF01926: MMR_HSR1" amino acids 13 to 139 (127 residues), 27.6 bits, see alignment E=6.7e-10 TIGR00231: small GTP-binding protein domain" amino acids 13 to 144 (132 residues), 70.5 bits, see alignment E=1.4e-23 PF03144: GTP_EFTU_D2" amino acids 309 to 375 (67 residues), 37.5 bits, see alignment E=6.5e-13 PF16658: RF3_C" amino acids 382 to 509 (128 residues), 141.7 bits, see alignment E=3.1e-45

Best Hits

Swiss-Prot: 69% identical to RF3_BORPE: Peptide chain release factor 3 (prfC) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 99% identity to bgf:BC1003_4666)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (533 amino acids)

>ABIE53_004717 peptide chain release factor 3 (Paraburkholderia graminis OAS925)
MSVSELKRRRTFAVISHPDAGKTTLTEKLLLFSGAIQIAGTVKGRKSNRYATSDWMEIEK
QRGISVASSVMQFEYGDAVINLLDTPGHEDFSEDTYRVLTAVDAAVMVIDGANGVEAQTL
KLLEVCRSRKTPIVTFINKLDREVREPLDLLDEIEQHLGVAAVPFTWPIGMGKDFQGVYD
IQRDQVRVFRAGQDKAGGDVETLQALSSEEGERRFGQSWVKAKEEIDLITGATPDFDREQ
FLAGQQSPVLFGSAINNFGVKEILDALVDLAPPPSMRMTVQRPVMPDEPKFTGVVFKVQA
NMDLAHRDRVAFIRVCSGRFERGMAVKVTRSNKTFRANNVVTFLSQRRETVSEAYPGDII
GIPNHGTLSLGDTLTEGEALQFVGLPFFAPEIFQTVEVVDPMRAKQLGEALKQLGEEGAI
QVFRPEVGGLMILGAVGQLQFEVVSHRLSTEYKVDVRMAPARYRMSRWVTCDDPAELRRF
TDSYAARIALDASDAPTYLASHVSEIEVAQKAWPKIVFNELREHSGAPFKKAM