Protein Info for ABIE53_003954 in Paraburkholderia graminis OAS925

Annotation: FMN-dependent oxidoreductase (nitrilotriacetate monooxygenase family)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR03860: FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family" amino acids 8 to 424 (417 residues), 518.7 bits, see alignment E=5.5e-160 PF00296: Bac_luciferase" amino acids 22 to 380 (359 residues), 184.8 bits, see alignment E=1.3e-58

Best Hits

Swiss-Prot: 57% identical to MOXC_BACSU: Putative monooxygenase MoxC (moxC) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 88% identity to bge:BC1002_3276)

MetaCyc: 57% identical to N-acetyl-S-alkylcysteine sulfoxide C-monooxygenase (Bacillus subtilis subtilis 168)
1.14.14.-

Predicted SEED Role

"Nitrilotriacetate monooxygenase component A (EC 1.14.13.-)" (EC 1.14.13.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.-

Use Curated BLAST to search for 1.14.13.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>ABIE53_003954 FMN-dependent oxidoreductase (nitrilotriacetate monooxygenase family) (Paraburkholderia graminis OAS925)
MSKKHIPFGIMLQGAGSHMNAWKHPAGPADASVNLDFMIRTAQKAEANGIAFAFVADGLY
INEKSIPHFLNRFEPISILSALATVTSKIGLAGTVSTSYSDPFTVARQFASLDLISGGRA
GWNVVTTPLEGTAKNYGGSHPEHALRYEIADEYLSVTQGLWDSWDDDAFVRDRARGVFFD
RDKLHPLNHQGKFFQVAGPLNIQRSLQGQPVIFQAGSSDAGIALAGKYADAVFTHSVSVE
ETRLFAQRVKDSAEAHGRSREDVKIFPGIGPIVGATHEEAEAKYRAIRDLLNIDEALAYL
GRFFDHHDFTQYPLDAPFPELGDLGRNSFRSTTDRIKANARSNGQTLREVALEVATPKTH
FLGTAEHVVDELIRWIEAGAADGFILGFAVQAQGLDDFVRYVIPELQRRGRYESTLSGAT
LRDHLGLPRKESRYTLTETA