Protein Info for ABIE53_003734 in Paraburkholderia graminis OAS925
Annotation: glutamate synthase (NADPH/NADH) large chain
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1212 amino acids)
>ABIE53_003734 glutamate synthase (NADPH/NADH) large chain (Paraburkholderia graminis OAS925) MVYKGLLLAGQVGVYYRDLQDERTVSALALVHQRFSTNTFPAWELAHPYRMIAHNGEINT VKGNVNWLNARTGAIASHVLGDDLPKLWPLIYPGQSDTASFDNCLELLVMAGYPLVHAMM MMIPEAWEQHTLMDDNRRAFYEYHAAMMEPWDGPAAIAFTDGRQIGATLDRNGLRPARYI VTDDDLVIMASEAGTLPIPESKIVKKWRLQPGKMFLIDMEHGRIIDDKELKDNLANAKPY KSWIDAVRIKLDEIEPKAEDVATERREAAALLDRQQAFGYTQEDLKFLMAPMAQAGEEAV GSMGNDSPLAVMSNKNKTLYHYFKQLFAQVTNPPIDPIRENMVMSLVSFVGPKPNLLDTN NINPPMRLEVSQPVLDFKDIAKIRAIDQYTGGKFSSYELNICYPVAWGKEGIEARLASLC AEAVDAVKSGYNMLIVSDRKTDRDNVAIPALLATAAIHTHLVQQGLRTSTGLVVETGSAR ETHHFALLAGYGAEAVHPYLAMETLAQLAAGLKGDLSAEKAIYNFTKAVGKGLQKVMSKM GISTYMSYTGAQIFEAVGLAEDLVQKYFKGTSSKVGGIGLFDVAEEAIRLHRDAFGDNPV LASMLDAGGEYAYRVRGEEHMWTPDAIAKLQHSARSNSYQTYKEYAHLINDQTKRHMTFR GLFEFKLDPAKAIPLDEVEPAKEIVKRFATGAMSLGSISTEAHATLAVAMNRIGGKSNTG EGGEDENRYRNELRGIPIKNGDTMKSVIGDEVIVDIPLKDGDSLRSKIKQVASGRFGVTA EYLASADQIQIKMAQGAKPGEGGQLPGHKVSDYIGKLRYSVPGVGLISPPPHHDIYSIED LAQLIHDLKNANPAASVSVKLVSESGVGTVAAGVAKAKADHVVIAGHDGGTGASPLSSIK HAGTPWELGLAETQQTLVLNQLRGRIRVQADGQMKTGRDVVIGALLGADEFGFATAPLVV EGCIMMRKCHLNTCPVGVATQDPVLRAKFKGQPEHVVNFFFFVAEEARELMAQLGIRKFD DLIGHAELLDMKKGVEHWKAKGLDFSRVFYQPQVPAEVARKHVEQQDHGLDRALDHTLIE KAKAAIEKGEHVSFIQPVRNVNRTVGAMLSGTIAKKYGHDGLPDDTIHIQLKGTAGQSFG AFLAKGITLDLVGDGNDYVGKGLSGRADHHSSHERFPRQVGREHHLRQHGDVRRDRRRSL LPRRGGRTFLRA